蜂毒素对SGC-7901细胞生长及G_2/M期阻滞的影响  

Effects of Melittin on the growth and G_2/M phase arrest in SGC-7901 cells

作　　者：吴宝明[1] 李俊[1] 吕雄文[1] 金涌[1] 赵斌[1] 黄艳[1] 王怡苹[1] 楼蓉[1] 王朋景[1] 

机构地区：[1]安徽医科大学药学院,安徽合肥230032

出　　处：《中国药理学通报》2010年第2期222-225,共4页Chinese Pharmacological Bulletin

基　　金：安徽省自然科学基金资助项目(No90413140);安徽高校省级优秀青年人才基金资助(No2009SQR2043);安徽省高校青年教师科研资助项目(No2008jq1067)

摘　　要：目的研究蜂毒素(Melittin)对胃上皮癌细胞SGC-7901细胞生长及细胞周期阻滞的影响。方法采用罗丹明B(SRB)染色法观察Melittin对SGC-7901细胞株生长曲线的影响;用流式细胞仪检测Melittin对该细胞周期阻滞的影响;RT-PCR检测细胞周期相关基因mRNA的表达。结果Melittin(1、2、4、8×10-3μg·L-1)体外给药24h,能抑制SGC-7901细胞的增殖活性(P<0.05orP<0.01);Melittin(4、8×10-3μg·L-1)作用SGC-790124h后,能明显阻滞该细胞株于G2/M期;并且降低G2/M期相关基因CylinB1-CDK1-Cdc25c mRNA的表达。结论Melittin具有抑制胃上皮癌细胞SGC-7901增殖的作用,其机制可能与干扰该细胞G2/M期相关基因的转录有关。Aim To explore the effects of the Melittin on growth and cell cycle of SGC-7901 cells. Methods Growth inhibition effect of Melittin was evaluated using SRB in SGC-7901 ceils in vitro;Melittin induced cell cycle arrest was investigated using flow cytometry assay; reverse transcription PCR（RT-PCR） was used to detect the associated protein mRNA of cell cycle. Resuits Proliferation activity of SGC-7901 cells was inhibited after treatment with Melittin（ 1,2,4,8,16,32 × 10^-3μ g. L^-1） （P 〈0. 05 or P〈0.01）for 24 h;Flowcytometry analysis revealed that SGC-7901 cells accumulated in the G2/M phase after treatment with Melittin（4,8 ×10-3 μg ·L^-1） for 24 h;the expression of CylinB1, CDK1 and Cdc25c mRNA were decreased. Conclutions Proliferation activity of SGC-7901 cells was inhibited by Melittin, which may be related to the inhibitory effect of Melittin on associated protein transcription in the G2/M stage of SGC-7901 cells.

关 键 词：蜂毒素 SGC-7901细胞 细胞增殖 细胞周期阻滞 CylinB1 CDK1 CDc25c 

分 类 号：R979.19[医药卫生—药品] R322.44[医药卫生—药学]