肿柄菊乙酸乙酯部位的化学成分  

Chemical constituent analysis of EtOAc fraction of Tithonia diversifolia(HEMSL.) A. Gray

作　　者：赵贵钧[1,2] 张崇禧[3] 吴志军[1] 李霞[1] 杨颖博[1] 孙连娜[1] 郑水庆[1] 

机构地区：[1]第二军医大学药学院生药学教研室,上海200433 [2]吉林农业大学中药材学院,长春130118 [3]山东大学威海分校海洋学院,威海264209

出　　处：《第二军医大学学报》2010年第2期189-192,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(20872177);上海市科委中药现代化专项资助项目(08DZ1971503)~~

摘　　要：目的肿柄菊乙酸乙酯部位化学成分的提取、分离和结构鉴定。方法肿柄菊地上部分80%的乙醇渗漉提取,通过萃取、硅胶柱层析、凝胶柱层析、MCI柱层析等方法进行成分分离,应用质谱、核磁等方法进行结构鉴定。结果从乙酸乙酯部位得到10个化合物,鉴定为香草醛(1)、麦角甾醇(2)、过氧化麦角甾醇(3)、3-甲氧基-4-羟基肉桂醛(4)、对羟基苯甲醛(5)、异东莨菪内酯(6)、5-羟甲基糠醛(7)、尼泊尔黄酮(8)、二十二烷酸单甘油酯(9)和13-羟基泽兰烯(10)。结论香草醛、麦角甾醇、过氧化麦角甾醇、3-甲氧基-4-羟基肉桂醛、对羟基苯甲醛、异东莨菪内酯、5-羟甲基糠醛、二十二烷酸单甘油酯和13-羟基泽兰烯均为首次从肿柄菊中分离得到。Objective To isolate and identify the chemical constituents of the EtOAc fraction of Tithonia diversifolia.Methods The dried aerial parts of Tithonia diversifolia were extracted with 80% EtOH,and the chemical constituents of the ethyl acetate portion from the extract were isolated by column chromatography with silica gel,Sephadex LH-20 and MCI.The isolated compounds were identified by NMR and MS analysis.Results Ten compounds were isolated from EtOAc extract,and identified as：vanillin（1）,ergosterol（2）,ergosterol peroxide（3）,3-methoxy-4-hydroxy-trans-cinnamaldehyde（4）,p-hydroxybenzaldehyde（5）,7-methylesculetin（6）,5-hydyoxymethyl furfural（7）,nepetin（8）,docosanoic acid 2,3-dihydroxypropyl ester（9） and 13-hydroxyeupatorochromene（10）.Conclusion Vanillin,ergosterol,ergosterol peroxide,3-methoxy-4-hydroxy-trans-cinnamaldehyde,p-hydroxybenzaldehyde,7-methylesculetin,5-hydyoxymethyl furfural,docosanoic acid 2,3-dihydroxypropyl ester,and 13-hydroxyeupatorochromene have been isolated from Tithonia diversifolia for the first time.

关 键 词：肿柄菊 化学成分 乙酸乙酯部位 

分 类 号：R931.6[医药卫生—生药学]