丹参-川芎水提取物有效组分配伍对大鼠心肌缺血／再灌注损伤的影响  

Effects of effective components compatibility of aqueous extracts of Salviae Miltiorrhizae （丹参 ） and Rhizoma Chuanxiong （川芎） on rat myocardial ischemia/reperfusion injury

作　　者：张大武[1] 刘剑刚[1] 丰加涛[2] 张蕾[1] 杨小平[2] 史大卓[1] 梁鑫淼[2] 

机构地区：[1]中国中医科学院西苑医院心血管病中心,北京100091 [2]中国科学院大连化学物理研究所,辽宁116023

出　　处：《中国危重病急救医学》2010年第2期109-112,共4页Chinese Critical Care Medicine

基　　金：中国科学院知识创新工程项目（KGCX2-SW-213）（

摘　　要：目的观察丹参-川芎水提取物有效组分配伍的注射液对大鼠心肌缺血／再灌注（I／R）损伤的影响。方法将SD大鼠按随机数字表法分为假手术组、模型组、冠心宁组及丹参-川芎低、高剂量组，每组10只。采用结扎冠状动脉左前降支建立1／R损伤模型。于结扎成功后10min股静脉注射给药，其中冠心宁组给予冠心宁注射液含生药2．88g／kg，丹参-川芎低、高剂量组分别给予丹参-川芎水提取物有效组分注射液含生药2．43g／kg和4．86g／kg；假手术组和模型组给予等量生理盐水。于缺血40min、再灌注120min取血后处死动物，测定血中肌钙蛋白T（cTnT）、肌酸激酶同工酶（CK—MB）、6-酮-前列腺素F1α（6-keto—PGF1α）、血栓素B2（TXB2）含量和血小板最大聚集率，并且测定心肌梗死程度。结果丹参-川芎低、高剂量组心肌梗死面积（（23．0±3．8）％、（20．8±4．7）％]较模型组[（29．1±3．2）％]显著降低（P〈0．05和P〈0．01）；cTnT[（0．78±0．29）mg／L、（O．76±0．29）mg／L3和CK—MB[（891．5±252．5）U／L、（759．5±191．3）U／L]也较模型组[（1．04±0．14）mg／L，（1268．2±256．5）U／L]显著降低（均P〈0．05）；丹参-川芎高剂量组6-keto—PGF1α较模型组升高[（206．7±35．6）ng／L比（138．6±28．9）ng／L，P〈0．053，血小板最大聚集率较模型组降低[（49．4±9．3）％比（77．1±16．7）％，P〈0．053。结论丹参-川芎水提取物有效组分配伍可减小心肌梗死面积，降低I／R后cTnT、CK—MB含量，提高6-keto—PGF1α/TxB。比值，从而减轻心肌I／R损伤。Objective To observe effects of effective components compatibility of aqueous extracts of Salviae Miltiorrhizae （丹参） and Rhizoma Chuanxiong （川芎） on myocardial ischemia/reperfusion （I/R） injury in rat. Methods Sprague-Dawley （SD） rats were randomly divided into sham group, model group, Guanxinning injection （冠心宁注射液） group and effective components of aqueous extracts from Salviae Miltiorrhizae and Rhizoma Chuanxiong salvianolic low dose group and high dose group, with 10 rats in each group. The myocardial I/R injury model was reproduced by ligation of the left anterior descending artery, and the experimental drugs were injected intravenously via femoral vein at 10 minutes after ligation. 2.88 g/kg Guanxinning injection was given in Guanxinning group, and 2.43 g/kg or 4. 86 g/kg effective components of aqueous extracts of Salviae Miltiorrhizae and Rhizoma Chuanxiong salvianolic was given in low dose group and high dose group, respectively, and equal volume of normal saline was given in sham group and model group. The anesthetic rats were sacrificed 40 minutes after ischemia and 120 minutes of reperfusion. Blood samples were collected before rats were sacrificed. The contents of serum troponin T （cTnT） and MB isoenzyme of creatine kinase （CK-MB） were determined, 6-keto-prostaglandin F1, （6-keto-PGF1α）, thromboxane B2 （TXB2） and platelet aggregation rate in blood plasma were assessed, and the degree of myocardial infarction in rats was determined. Results The myocardial infarction size in combined Salviae Miltiorrhizae and Rhizoma Chuanxiong low dose group and high dose group [（23.0 ± 3.8）%, （20. 8±4.7）%] were lower significantly than that in model group [（29.1±3.2）%, P〈0. 05 and P〈0.01], the contents of serum cTnT [（0. 78±0. 29） mg/L, （0.76±0.29） mg/L3 and CK-MB （（891.5± 252.5） U/L, （759.5 ± 191.3） U/L] were lower significantly than those in model group [（1.04±0. 14） rag/L, （1 268.2±256.5） U/L, all P〈0. 05].

关 键 词：缺血/再灌注损伤 心 丹酚酸B 川芎酚酸 6-酮-前列腺素F 1α 血栓素 血小板聚集率 

分 类 号：R285.5[医药卫生—中药学]