胶原、壳聚糖包覆的小单层脂质体的体外稳定性评价  

EVALUATION OF IN VITRO STABILITY OF SMALL UNILAMELLAR VESICLES COATED WITH COLLAGEN AND CHITOSAN

作　　者：史向阳[1,2] 孙曹民[1,2] 吴世康[1,2] 

机构地区：[1]中国科学院感光化学研究所 [2]北京理工大学生物材料实验室

出　　处：《生物物理学报》1998年第4期635-641,共7页Acta Biophysica Sinica

基　　金：国家自然科学基金

摘　　要：为了提高小单层脂质体（ＳｍａｌＵｎｉｌａｍｅｌａｒＶｅｓｉｃｌｅｓ，ＳＵＶ）的体外稳定性，以５（６）－羧基荧光素（５（６）－ＣＦ）为荧光探针，研究了脂质体被胶原蛋白和壳聚糖这两种天然生物大分子包覆后的通透性。发现，胶原蛋白、壳聚糖和磷脂酰胆碱（ＰＣ）的重量比分别为２：１和８：１时，已能明显降低脂质体的通透性。并以一种分子内电荷转移化合物３－甲氧基－４＇－Ｎ，Ｎ－二甲氨基黄酮（ＤＭＭＦ）为探针，用荧光偏振法研究了胶原蛋白、壳聚糖和ＰＣ在上述比例下，对脂质体膜流动性的影响，发现脂质体在被包覆前后，其膜的流动性没有明显的改变。可见，胶原蛋白、壳聚糖包覆脂质体能够在基本不干扰脂质体膜流动性的情况下。To improve the stability of small unilamellar vesicles(SUV) in vitro, the permeability of SUV from soybean phosphatidylcholine (PC) coated by collagen and chitosan was studied respectively by using 5(6)-Carboxyflourescein(5(6)-CF) as a fluorescence probe. Results showed that coating with collagen/PC weight ratio of 2 or chitosan/PC weight ratio of 8 decreased significantly the permeability of the liposomal membrane. Moreover, fluorescence polarization method was used to study the influence of coating with collagen and chitosan at the above ratio to PC on the fluidity of the liposomal membrane, by using an intramolecular charge transfer compound 3-methoxy-4'-N, N-dimethylamino flavone derivative(DMMF) as a fluorescence probe. Results showed that the fluidity of the liposomal membrane after coating with the two macromolecules had no obvious changes. It indicates that SUV coated with collagen and chitosan at appropriate weight ratio can significantly improve the stability of the liposomal membrane in vitro without disturbing its fluidity.

关 键 词：胶原 壳聚糖 脂质体 稳定性 

分 类 号：Q54[生物学—生物化学] Q734