黄芪多糖抗肿瘤活性研究  

Antitumor activity of Astragalus Polysaccharides

作　　者：任美萍[1] 刘明华[1] 李蓉[1] 肖顺汉[1] 李华[2] 李茂[1] 

机构地区：[1]泸州医学院药理毒理研究室,泸州646000 [2]泸州医学院药理学教研室,泸州646000

出　　处：《中国新药杂志》2010年第3期221-224,共4页Chinese Journal of New Drugs

基　　金：四川省教育厅重点项目(2005A076)

摘　　要：目的:研究黄芪多糖的抗肿瘤活性及对荷瘤小鼠血清中IL-2,TNF-α水平的影响。方法:以人肿瘤细胞株为模型,采用cell counting kit-8(CCK-8)检测体外抗肿瘤活性;以H22和S180为模型,检测体内抗肿瘤活性;ELISA法检测对荷瘤小鼠血清中IL-2和TNF-α水平的影响。结果:黄芪多糖对K562,SMMC-7721,HCT116和A549细胞的增殖无明显影响,在50,100 mg.kg-1时,对H22的抑瘤率分别为32.84%,45.09%,对S180的抑瘤率分别为36.55%,50.35%,且能提高荷瘤小鼠血清中IL-2和TNF-α的水平。结论:黄芪多糖无明显细胞毒活性,但体内抗肿瘤活性较强,其机制与增强机体的免疫功能有关。Objective： To study the antitumor activity of Astragalus Polysaccharides and the levels of IL-2 and TNF-α in tumor-bearing mice. Methods：The cytotoxicity on human tumor cell lines was determined by cell counting kit-8 （CCK-8） assay. The inhibitory effect on tumor growth was observed by the models of hepatoma 22 （H22） and implanted sarcoma 180 （S180） in mice. The levels of IL-2 and TNF-α in tumor-bearing mice were investigated by ELISA assay. Results：Astragalus Polysaccharides had no effect on the proliferation of K562, SMMC- 7721, HCT116 and A549 cells. At doses of 50 and 100 mg· kg ^-1 ,the inhibitory rates of Astragalus Polysaccharides on H22 and S180 were 32.84% , 45.09% and 36.55% , 50.35% , respectively. Astragalus Polysaccharides enhanced the expression levels of IL-2 and TNF-α in tumor-bearing mice. Conclusion： Astragalus Polysaccharides had no cytotoxicity, but exhibited anti-tumor effect in vivo, and the mechanism may be related to enhancing the immune function.

关 键 词：黄芪多糖 抗肿瘤 白介素-2 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学] R965[医药卫生—中医学]