白附子木脂素化合物调节人胃癌SGC-7901细胞TRAIL及其受体的表达  

Study of lignans of Rhizoma Typhonii on the expression of TRAIL and its receptors in gastric carcinoma cell line(SGC-7901)

作　　者：马英丽[1] 李艳凤[1] 唐丽萍[1] 艾凤伟[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中国新药杂志》2010年第3期225-228,共4页Chinese Journal of New Drugs

基　　金：黑龙江省自然科学基金(D200630)

摘　　要：目的：探讨白附子木脂素化合物对人胃癌细胞株（SGC-7901）的TRAIL及其受体TRAIL—R1和TRAIL—R2表达的影响。方法：SGC-7901细胞在不同浓度组0．026～26μg·L^-1的白附子木质素化合物中培养24h后，用MTT法检测白附子木脂素化合物对细胞增殖的抑制作用；利用Hoechst33342／PI染色法观察细胞凋亡；采用RT—PCR方法检测不同浓度白附子木脂素化合物对SGC-7901细胞TRAIL及其受体mRNA基因表达。结果：不同浓度组0．026～26μg·L^-1的白附子提取物对SGC-7901细胞增殖的抑制率分别为7．35％，16．11％，27．18％和50．58％；且均导致细胞凋亡的改变；同时其TRAIL及其受体TRIAL—R1和TRAIL—R2 mRNA表达水平明显高于空白对照组。结论：白附子木脂素化合抑制了SGC-7901的增殖并诱导其凋亡。作用机制可能与上调TRAIL及其受体TRAIL—R1和TRAIL-R2有关。Objective ： To study the effect of lignans of Rhizoma Typhonii on the expression of TRAIL and its receptors in gastric carcinoma cell line （ SGC-7901 ）. Methods ： The proliferation of SGC-7901 cells was measured by MTT assay after exposure to extracts of Rhizoma Typhonii at concentration 0. 026 ～26μg·L^-1 for 24 h. The morphology of cell apoptosis was observed by Hoechst33342 fluorescent staining. TRAIL and TRAIL-Rs mRNA expression in SGC-7901 cells were determined by RT-PCR. Results： The inhibition rate was 7.35% , 16. 11% , 27. 18 % and 50.58% at the different concentration 0. 026 ～26μg·L^-1 extracts of Rhizoma Typhonii, respectively. Interestingly, the expression of TRAIL,TRAIL-R1 and TRAIL-R2 was significantly higher in the experiment groups at concentrations than control group. Conclusions： The up-regulation of TRAIL/TRAIL-R1 and TRAIL-R2 by Lighans of Rhizoma Typhonii could be involved in the induction of apoptosis in SGC-7901 ceils.

关 键 词：白附子 木脂素成分 SGC-7901细胞 细胞凋亡 TRAIL 

分 类 号：R282.71[医药卫生—中药学] R979.1[医药卫生—中医学]