闭锁繁育乌骨鸡群体的RAPD指纹分析  

Randomly Amplified Polymorphic DNA Fingerpinting Analysis for a Close bred Population of Black boned Chickens

作　　者：吴晓林[1,2,3] 肖兵南 蒋隽[1,2,3] 刘荣宗 施启顺[1,2,3] 柳小春[1,2,3] 戴英红 

机构地区：[1]湖南省畜牧兽医研究所家禽育种中心 [2]湖南农业大学动物科技学院 [3]湖南省洪江市畜牧水产局

出　　处：《生命科学研究》1998年第4期278-282,共5页Life Science Research

基　　金：湖南省自然科学基金;湖南省"九五"重点攻关计划

摘　　要：随机抽样测定了一个长期闭锁繁育和一个开放繁育雪峰乌骨鸡群体的早期生长和成活率，并藉ＲＡＰＤ分析比较了２个乌骨鸡群体的遗传学特征，结果表明：闭锁繁育群体ＲＡＰＤ标记的平均共带系数和平均带纹频率显著高于开放群体．标记效应估计结果表明随机引物Ｓ１０２扩增的ｂ４（１８２０ｂｐ）和ｂ５（１９３０ｂｐ）片段和Ｓ１０５扩增的ｂ９（１８４９ｂｐ）和ｂ１０（４１０４ｂｐ）片段作为遗传标记对于体重（尤其是３５日龄和７０日龄体重）具有很高的负向相关效应，因此，该４个ＲＡＰＤ标记可以作为近交衰退候选指示标记加以深入研究，最终结论有待于在大样本、多群体、多世代资料中验证．Early growth and survival rate were examined randomly in a close bred and an open bred populations of Xuefeng Black boned Chickens.A comparison of RAPD profiles was subsequently made between these two populations regarding average band sharing (ABS) and average band frequency (ABF).The results indicted the presence of significantly greater ABS and ABF in the close bred population than in the open bred population.Estimation of marker effects with a generalized linear model suggested that the fragments b 4 (1 820 bp) and b 5(1 930 bp) amplified by the oligonucletide primer S102,and the fragment b 9 (1 849 bp) and b 10 (4 104 bp) amplified by the primer S105 could possibly be indicator markers for inbreeding depression if further verified with data in a variety of populations of at least more than one generation.

关 键 词：乌骨鸡 近交衰退 RAPD 体重 闭锁繁育 

分 类 号：S831.2[农业科学—畜牧学]