百合鳞片细胞形态发生中生理生化特性研究  

Studies on the Changes and Comparison of Physiology and Biochemistry in the Morphogenesis of Scale Explant of Lily in vitro

作　　者：屈姝存[1] 刘选明[1] 周朴华[1] 卢向阳[1] 罗泽民[1] 

机构地区：[1]湖南农业大学理学院

出　　处：《生命科学研究》1998年第4期288-294,共7页Life Science Research

基　　金：湖南省科委自然科学基金

摘　　要：四倍体百合鳞片外植体脱分化形成愈伤组织过程其蛋白质、核酸含量与蛋白酶、淀粉酶、过氧化物酶ＰＯＤ、酸性磷酸酯酶活性先升高后降低，淀粉与ＡＴＰ含量呈降－升－降变化，ＳＯＤ活性先降低后升高，愈伤组织分化芽过程，除ＡＴＰ含量降低外，其余指标均不断升高．鳞片外植体直接分化发育不定芽过程中，仅见蛋白质、淀粉、ＡＴＰ含量与ＳＯＤ、酸性磷酸酯酶活性在５ｄ前降低，其它时间各项指标均升高，直到不定芽发育完成再下降．器官发生型比器官型经历了更为深刻的内部生理生化变化．The results indicated:the content of protein,uncleic acid and the activity of protease,amylase,POD and acidic phosphatase increased before the callus formation,and the activity of SOD changed contrany to this in the same time,but the content of starch and ATP decreased in initial stage,then increased during the callus formation and decreased again after the callus formation.During the bud differentiation from calli,all targets increased except the ATP content.In organogeny type morphogenesis,the content of protein,starch,ATP and the activity of SOD,acidic phosphatase decreased only before the first five days,the other targets increased in the same time.During the period of the direct bud formation ,all targets increased.After the bud formation,those target all became to fall.The changes of physiology and biochemistry in cells were greater in organogeny type morphogenesis than in organ type morphogenesis.

关 键 词：百合 器官型 器官发生型 形态发生 生理生化 

分 类 号：Q944.5[生物学—植物学]