糖皮质激素快速抑制牛蛙椎旁神经节B细胞快兴奋性突触后电位  

RAPID EFFECT OF GLUCOCORTICOID ON EXCITATORY POSTSYNAPTIC POTENTIAL OF B CELLS IN BULLFROG PARAVERTEBRAL GANGLIA

作　　者：马蓓[1] 易必达[2] 邢宝仁[1] 

机构地区：[1]第二军医大学生理教研室,上海200433 [2]贵阳医学院生理教研室,贵阳500045

出　　处：《生理学报》1998年第6期679-682,共4页Acta Physiologica Sinica

基　　金：军队九.五医药卫生基金!96Z035

摘　　要：用单个方波电刺激牛蛙离休椎旁神经节节前纤维，细胞内记录节后B细胞快兴奋性突触后电位（f－EPSP），观察糖皮质激素（GC）对B细胞f－EPSP，的快速抑制作用。结果发现，GC灌流3min，B细胞f－EPSP的幅值减小，撤除GC后，EPSP的幅值恢复到对照水平，作用具有剂量依赖性。糖皮质激素胞内受体阻断剂RU38486能阻断GC的快速抑制作用。蛋白质合成抑制剂放线菌酮不能阻断该快速作用。结果提示，GC对B细胞兴奋性突触后电位的快速抑制是通过非基因机制起作用的。In the present study, the rapid effect of hydrocortisone 21-hemisuccinate (F-suc) on the amplitude of f-EPSP of B cells in isolated bullfrog symathetic ganglia was examined with the use of intracellular recording technique. It was found tha the amplitude of f-EPSP of B cells was decreased by F-suc, with a latency less than 3 min after the beginning of perfusion of F-suc. The inhibition of F-suc was concentration-dependent and could be blocked by the antagonist of intracellular glucocorticoid receptor RU38486, but not by the protein synthesis inhibitor actidione. These findings suggest that the inhibition of f-EPSP of B cells by F-suc is probably mediated through nongenomic mechanism.

关 键 词：糖皮质激素 椎旁神经节 f-EPSP 

分 类 号：Q577[生物学—生物化学] R338.8[医药卫生—人体生理学]