生防木霉菌耐三唑类杀菌剂菌株的选育  

Selection of Trichoderma Mutants Resistant to Triazole Fungicides

作　　者：梁志怀[1,2] 魏林 安哲宇[3] 陈玉荣 

机构地区：[1]湖南农业大学,长沙410128 [2]湖南省植物保护研究所,长沙410125 [3]中南大学研究生院隆平分院,长沙410125

出　　处：《中国生物防治》2010年第1期60-65,共6页Chinese Journal of Biological Control

基　　金：湖南省博士后科研资助专项计划项目(2008RS4028);国家"十一五"科技支撑计划项目(2006BAD17B08)

摘　　要：应用含药培养基诱导和紫外线诱变相结合的方法,对优良生防木霉菌株T2-16进行了抗三唑类杀菌剂的遗传改良,获得了具有较好耐药性突变体13株。通过测定突变株对水稻纹枯病菌(Rhizoctonia solani)、稻瘟病菌(Magnaporthe oryzae)和稻曲病菌(Ustilaginoidea virens)的拮抗性,获得1株拮抗活性高、对三唑类杀菌剂耐性好的突变体TUV-13,其对腈菌唑抗性提高10倍,对三唑酮抗性提高51倍,且耐药性可以稳定遗传。The biocontrol Trichoderma strains were genetically improved by U.V.irradiation and selection on PDA medium containing fungicides.Thirteen mutation strains with higher tolerance against Myclobutanil were obtained.Among them,strain TUV-13 showed highest antagonistic activity against Rhizoctonia solani,Magnaporthe oryzae and Ustilaginoidea virens.The tolerance of strain TUV-13 to Myclobutanil and Triadimefon increased 10 times and 51 times in comparison with that of the wild type strain T2-16,respectively.The result also showed that the resistance did not decline after transference of culture ten times continuously on PDA plates.

关 键 词：木霉 紫外线诱变 腈菌唑 耐药性 水稻病害 

分 类 号：S482.292[农业科学—农药学] Q933[农业科学—植物保护]