盐地碱蓬内生中度嗜盐菌的分离与系统发育多样性分析  

Phylogenetic Diversity of Endophytic Moderately Halophilic Bacteria Isolated from Suaeda salsa L.

作　　者：崔春晓[1] 戴美学[1] 夏志洁[1] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014

出　　处：《微生物学通报》2010年第2期204-210,共7页Microbiology China

基　　金：山东省自然科学基金项目(No.Y2007D40);山东大学微生物技术国家重点实验室开放课题基金项目

摘　　要：为了了解东营滨海盐地碱蓬植株内生中度嗜盐菌的多样性,采用传统分离鉴定技术和基于16S rRNA序列分析对样品中可培养细菌的多样性进行研究。根据其生理生化特征、16S rRNA序列测定和系统发育分析,分离获得的15株内生菌可分为4个类群,涉及Halomonadaceae科的Chromohalobacter属、Kushneria属、Halomonas属以及Bacillaceae科的Bacillus属。类群I中4菌株的16S rRNA序列与Chromohalobacter israelensis的最高相似性为95%。类群II共7株菌,归属于Kushneria属,是碱蓬内生中度嗜盐菌中的优势类群。类群III菌株的16S rRNA序列与一株尚无明确分类地位的Gammaproteobacteria亚门耐盐固氮细菌Haererehalobacter sp.JG11的相似性为99%。类群IV中的芽孢杆菌的16S rRNA序列与已知细菌的相似性为96%,很可能代表了Bacillus属的新种。各种水解酶类的分析表明,在分离的15株菌中有3株菌产蛋白酶,14株产酯酶,8株产DNA酶,11株产半乳糖苷酶,14株产脲酶。研究结果揭示,盐地碱蓬中存在较为丰富的中度嗜盐菌多样性和系统发育多样性,并且潜藏着较多的新的微生物类群。The traditional isolation and identification method and analysis based on 16S rRNA sequences were used to investigate the phylogenetic diversity of endophytic moderately halophilic bacteria of Suaeda salsa L. in Dongying. According to physiological and biochemical characteristics, 16S rRNA sequences and phylogenetic analysis, 15 isolated endophytic bacterial strains were classified into four groups, belonging to genera Chromohalobacter, Kushneria, Halomonas or Bacillus separately. 16S rRNA sequences of 4 strains in Group Ⅰ had the highest similarity to Chromohalobacter israelensis （95%）. Group Ⅱ which contained 7 strains of the genus Kushneria is the dominant group in Suaeda salsa L.. The strains in Group Ⅲ bear the closest affiliation to Haererehalobacter sp. JG 11 （99%）, which is not classified definitely. The only endospore-forming bacterium was classified into group IV. Its 16S rRNA sequence similarities with respect to the known Bacillus species were ≤ 96%, which was presumed to be potential novel species. Among these isolated strains, 3 strains produced proteinase; 14 strains produced esterase; 8 strains produced DNase; 11 strains produced galactosidase; 14 strains produced urease. The research results indicated that there was not only abundant phylogenetic diversity of moderately halophilic bacteria, but also some unknown bacteria groups existed in Suaeda salsa L..

关 键 词：中度嗜盐菌 盐地碱蓬 系统发育分析 

分 类 号：Q93[生物学—微生物学]