抗菌肽Fengycins抑制串珠镰刀菌的初步机制  

Mechanism of Inhibition to Fusarium moniliforme by Antimicrobial Peptide Fengycins

作　　者：胡梁斌[1,2] 周威[1] 章挺[3] 杨志敏[4] 徐剑宏[1] 石志琦[1] 

机构地区：[1]江苏省农业科学院食品质量安全与检测研究所,江苏南京210014 [2]河南科技学院食品学院,河南新乡453003 [3]江西省林业科学院,江西南昌330032 [4]南京农业大学生命科学学院,江苏南京210095

出　　处：《微生物学通报》2010年第2期251-255,共5页Microbiology China

基　　金：江苏省国际科技合作项目(No.BZ2005045)

摘　　要：Fengycins是枯草芽孢杆菌非核糖体合成的环状脂肽类抗生素,本文从其特性入手,研究了其抑制串珠镰刀菌的初步作用机制。普通显微观察结果显示,Fengycins处理能使部分串珠镰刀菌菌丝顶端破裂,进一步通过PI染色与荧光显微观察发现,Fengycins处理会导致串珠镰刀菌菌丝膜的损伤。在添加几丁质、壳聚糖、β-1,3葡聚糖、甾醇、胆固醇的平板内,Fengycins的抑菌活性没有受到太大影响;而在添加卵磷脂的平板,Fengycins的抑菌活性受到明显的拮抗。这些结果说明卵磷脂很可能是Fengycins在膜上的作用靶标。此外,研究还发现Fengycins能够抑制串珠镰刀菌分泌的磷脂酶A2的活性,该性质很可能也在Fengycins的抑菌活性中起到了一定作用。Fengycin is a cyclic lipopeptide antibiotic produced nonribosomally by Bacillus subtilis. In this study, the primary mechanism of inhibition to Fusarium moniliforme by Fegycins was investigated according to their special properties. Microscopic observation on morphology of E moniliforme mycelia revealed that tips of partial mycelia were lysed. PI staining of Fengycins-treated F. moniliforme mycelia was observed under fluorescence microscope, which indicated the toxin-mediated damage of cytoplasmic membrane. Further study showed that addition of chitin, chitosan, 13-1,3-glucan, ergosterol and cholesterol in agar media could not significantly modify the inhibition to F. moniliforme by Fengycins, while addition of phosphatidylcholine could antagonize the antifungal activity of Fengycins. These results suggested that phosphatidylcholine probably was the plasma membrane target for Fengycins. Additionally, our results also showed that fengycins could depress the activity of PLA2 secreted by F. moniliforme, which might contribute to the antifungal activity of fengycins against F. moniliforme. Keywords： Fengycins, Fusarium moniliforme, Mechanism of inhibition

关 键 词：Fengycins 串珠镰刀菌 抑制机制 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]