E-选择素突变体的构建和表达  

Cloning and expression of E selectin mutants

作　　者：胡美茹[1] 蔡铀庆[1] 孙英勋[1] 于鸣[1] 裴武红[1] 汲言山[1] 沈倍奋[1] 

机构地区：[1]军事医学科学院基础医学研究所

出　　处：《细胞与分子免疫学杂志》1998年第4期247-248,共2页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家"863"青年基金

摘　　要：从ｐＵＣ１８／Ｅ－选择素（Ｅ－ｓｅｌｅｃｔｉｎＣＤ６２Ｅ）重组质粒，以ＰＣＲ的方法扩增得到Ｅ－ｓｅｌｅｃｔｉｎ突变体ＣＤ６２Ｅ１和ＣＤ６２Ｅ２基因。将其分别插入痘苗病毒表达载体ｐＪＳＡ１１７５的单一ＳｍａⅠ位点，在痘苗病毒真核系统中进行了表达。初步的细胞粘附功能试验结果表明，Ｅ－ｓｅｌｅｃｔｉｎ分子不同结构域的缺失可削弱其粘附能力，为进一步研究Ｅ－ｓｅｌｅｃｔｉｎ公子的结构与功能奠定了基础。Two E selectin mutant genes, CD62E1 and CD62E2, were amplified by PCR from our constructed plasmid pUC18/E selectin (CD62E). The expression plasmids were obtained by inserting the two mutant genes into pJSA1175, which were expressed in vaccinia virus system. U937 cell adhesion assay showed that deletion of EGF domain or EGF+CR 1+CR 2 domains weakened the cell adhesion mediated by E selectin molecules. These findings provided a clue to the mechanisms of some diseases and their treatment. It is very useful for investigating the relationship between the structure and function.

关 键 词：E-选择素 疫苗病毒 突变体 表达 构建 

分 类 号：Q78[生物学—分子生物学] R392.11[医药卫生—免疫学]