DNA免疫引起小鼠产生抗丙型肝炎病毒核壳区体液应答的初步研究  

DNA BASED IMMUNIZATION FOR THE INDUCTION OF HUMORAL RESPONSE AGAINST THE HEPATITIS C VIRUS NUCLEOCAPSID IN MICE

作　　者：谭文杰[1] 丛郁[1] 毕胜利[1] 苗季[1] 丛旭[1] 詹美云[1] 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《中国免疫学杂志》1998年第6期453-455,共3页Chinese Journal of Immunology

基　　金：中国预防医学科学院病毒学研究基金

摘　　要：采用ｐｃＤＮＡ３与ｐＭＴ两种基因表达裁体，构建包含丙型肝炎病毒（ＨＣＶ）核心区（Ｃ区）或全结构基因区（核心区、包膜区）的质粒。经纯化后直接对Ｂａｌｂ／ｃ小鼠进行骨骼肌或尾部皮内注射，２～６ｗ后采血，用ＥＬＩＳＡ法检测小鼠抗核壳区的体液应答。结果表明：ＤＮＡ免疫可引起小鼠产生抗ＨＣＶ核壳区的体液应答，加强免疫２次后可使特异性抗体平均滴度达到１８００以上。提示ＤＮＡ免疫可作为丙型肝炎病毒感染的潜在防御手段。Plasmids which contains hepatitis C virus nucleocapsid region alone or structural gene were constructed by cloning into two eukaryotic expression vector(pcDNA3,pMT).Intramuscular or intradermal immunization of Balb/c mice with these constructs in the form of naked DNA elicited humoral responses to the HCV nucleocapsid within 2 to 6 weeks postinjection in enzyme linked immunosorbent assay.The mean serum titer anti nucleocapsid specific antibody were found to be above 1∶800 after two boosted injections,and these titers remained stable until the end of the study(18 weeks postinjection).This findings suggest the HCV structural gene immunization warrants further investigation as a potential vaccine against HCV infection.

关 键 词：丙型肝炎病毒 DNA免疫 体液应答 

分 类 号：R512.630.1[医药卫生—内科学]