鲤正、反交F_2群体的AFLP遗传图谱构建及其QTL定位  

AFLP Genetic Map Construction and QTLs Identification from F_2 Reciprocal Populations in Common Carp,Cyprinus carpio L.

作　　者：王军[1] 王成辉[1] 刘豪[1] 马玉清[1] 杨新鑫[1] 

机构地区：[1]上海海洋大学农业部水产种质资源与利用重点开放实验室,上海201306

出　　处：《中国农业科技导报》2010年第1期92-99,共8页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(30700622);上海市教委项目(07ZZ136);上海市重点学科建设项目(Y1101)资助

摘　　要：以高代选育的荷包红鲤和兴国红鲤的正、反交F2群体为材料,利用AFLP标记构建了鲤正、反交群体的遗传图谱,并进行了生长相关性状的QTL定位。在正交群体中,14对AFLP引物共产生542个多态性标记,其中325个标记符合3∶1的孟德尔分离比例,利用其构建的遗传图谱涵盖50个连锁群,图谱总长度3 676.1 cM,并获得了与全长、体长、体高、尾柄长和尾柄高5个生长性状相关的17个QTL,可解释表型变异的1.66%-70.49%;在反交群体中,14对AFLP引物共产生605个多态性标记,其中333个标记符合3∶1孟德尔分离比例,构建的遗传图谱也涵盖50个连锁群,图谱总长度3 943.1 cM,获得了与体重、全长、体长、体高和尾柄长5个生长性状相关的15个QTL,可解释表型变异的1.58%-63.89%。还对正、反交群体构建的遗传图谱及QTL定位差异及结果进行了初步分析和探讨。F2 reciprocal populations derived from a diallel cross of Purse red common carp and Xingguo red common carp was used as material,genetic maps in the common carp（Cyprinus carpio L.） was constructed using AFLP marker and the growth-related quantitative trait loci（QTL） was identified.In positive mating population,542 polymorphic markers were produced from 14 pairs of AFLP primers,in which 325 markers were accord with the ratio of 3∶1.A genetic map containing 50 linkage groups were constructed with genome length of 3 676.1 cM,and 17 QTLs related to total length,standard length,pre-dorsal height,caudal peduncle length and caudal peduncle height were achieved,which could explain 1.66% to 70.49% of the total variation.In negative mating population,605 polymorphic markers were produced with 14 pairs of AFLP primers,in which 333 markers were accord with the ratio of 3∶1.Another genetic map containing 50 linkage groups were also constructed with genome length of 3 943.1 cM,and 15 QTLs related to body weight,total length,standard length,pre-dorsal height,caudal peduncle length were also gained,which could explain 1.58% to 63.89% of the total variation.Furthermore,this paper carried out initial analysis and discussion on genetic maps constructed from reciprocal population,the differences between QTL identification and their results.

关 键 词：鲤 正反交F2群体 AFLP标记 遗传图谱 QTL 

分 类 号：S917.4[农业科学—水产科学]