青藤碱对体外人外周血单个核细胞肿瘤坏死因子α表达的影响  

Effect of Sinomenine on Expression of Tumor Necrosis Factor Alpha of Human Peripheral Blood Mononuclear Cells in Vitro

作　　者：接力刚[1] 杜红延[2] 沈鹰[1] 黄清春[1] 孙维峰[1] 韦嵩[1] 

机构地区：[1]广州军区广州总医院中医科,广州510010 [2]南方医科大学生物技术学院

出　　处：《中国临床保健杂志》2010年第1期21-23,F0003,共4页Chinese Journal of Clinical Healthcare

基　　金：全军医学科技研究"十一五"计划面上项目(06MB159);广东省中医药局科技项目(103151)

摘　　要：目的观察青藤碱对体外培养人外周血单个核细胞TNF-α表达的影响。方法将分离的正常人外周血单个核细胞分成5组,分别给予0.9%氯化钠注射液、高剂量青藤碱、中剂量青藤碱、低剂量青藤碱和地塞米松;孵育48 h后,提取细胞培养液上清液,用放免法检测肿瘤坏死因子α的含量,用半定量RT-PCR法检测外周血单个核细胞中TNF-αmRNA的表达。结果高剂量青藤碱组和地塞米松组细胞培养液的上清液TNF-α含量明显低于0.9%氯化钠注射液组(P<0.05),高剂量青藤碱组的TNF-α的含量水平明显低于中、小剂量青藤碱组(P<0.05),且其外周血单个核细胞TNF-α的mRNA表达水平较0.9%氯化钠注射液组有显著下降(P<0.05)。结论抑制人外周血单个核细胞表达TNF-α可能是青藤碱治疗强直性脊柱炎的机制之一。Objective To observe the effect of sinomenine on the expression of tumor necrosis factor alpha （TNF-α） of Human Peripheral Blood Mononuclear Cells （PBMC） in Vitro. Methods The human PBMC were derived from healthy volunteer＇ s vein blood, and were divided into five groups. Them were respectively treated with normal sodium,large dose compound sinomenine, middle dose sinomenine, low dose sinomenine, dexamethasone, and then cultured for 48 h. The level of TNF-α supernatant were tested by radioimmunity, the expression of TNF-αmRNA of PBMC was measured by semi-quantitative RT-PCR respectively. Results The level of TNF-α in supernatant from large dose sinomenine group and dexamethasone group was remarkably lower than from normal sodium group （ P 〈 0.05 ）. The level of TNF-α in supernatant in large dose sinomenine group was markedly lower than in middle and low dose sinomenine groups. Compared with normal sodium group, the mRNA expression of TNF-α markedly decreased in high dose sinomenine group （ P 〈 0.05 ）. Conclusion To inhibit expression of TNF-α of human PBMC may be one of mechanisms of sinomenine in treating ankylosing spondylitis.

关 键 词：脊柱炎 强直性 肿瘤坏死因子Α 病理过程 

分 类 号：R285.5[医药卫生—中药学]