庚型肝炎病毒同丙型肝炎病毒的联合感染和单独感染  

HGV Infection and Coinfection of HGV with HCV

作　　者：雷金娥[1] 李和平[1] 钟晓红[1] 郭清芳[1] 王雯莉[2] 

机构地区：[1]西安医科大学第一临床医学院检验科,710061 [2]卫生部临床检测中心

出　　处：《临床肝胆病杂志》1998年第4期222-224,共3页Journal of Clinical Hepatology

摘　　要：为了建立HGV感染的酶免疫诊断方法，用计算机技术分析了HGV序列，预测核心区，NS3区的抗原表位，合成了多段的HGV核心和NS3区合成肽，通过免疫筛选试验，证实C29，C40和N310三段肽有免疫原性。用这三段肽包被酶标板，用间接免疫试验原理，建立了HGV的EIA检测方法。用中和抑制试验检测了该方法的特异性。使用HGVEIA方法检测卫生部临床检测中心提供的HCVEIA参比血清，发现3／92抗－HCV阳性和12／58抗－HCV阴性标本抗-HGV抗体阳性。使用RT—PCR扩增HGV的5’非编码区，在15’份抗－HGV阳性标本中仅有2份HGVRNA阳性。以上结果提示使用EIA方法比HGVRT—PCR方法有更高的HGV感染检出率，抗－HGV抗体阳性仅表示HGV感染，HGVRNA阳性表明病毒复制。在我国献血员中存在HGV的单独感染和HGV同HCV的联合感染。Some oligopeptides from HGV genome had been synthetied. C29 and C44 peptides coded in core and N310 peptides coded in NS3 of HGV were selected asimmobilizing antingen. 150Sera detected were from a panel for detecting antibody againist HCV. Antibody to HCV was detected in 3 of 92 samples with antibody to HCV and 12 of 58 without antibody to HCV. In 15 samples with antibody to HGV, only,two was detectable for HGV RNA by nest RT-PCR. The clinical significance of HGV single infection and coinfection of HGV and HCV needs to explore.

关 键 词：庚型肝炎 丙型肝炎 联合感染 

分 类 号：R512.63[医药卫生—内科学]