5-FU和放射线联合应用对人子宫颈腺癌HeLa细胞凋亡的影响  

Effect of 5-Fluorouracil and Radiotherapy on the Apoptosis of Human Cervical Adenocarcinoma HeLa Cells

作　　者：杨滨[1] 蔡敏[1] 阎华 邹华伟[1] 杨帆[1] 刘渊[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004 [2]辽宁省计划生育科学研究院附属医院,沈阳110031

出　　处：《中国医科大学学报》2010年第1期24-27,共4页Journal of China Medical University

摘　　要：目的研究5-氟尿嘧啶(5-FU)和放射线联合应用对人子宫颈腺癌HeLa细胞凋亡的影响。方法将人子宫颈腺癌HeLa细胞分为单纯5-FU化疗组、单纯放射线组、放化疗联合组(5-FU和放射线联合应用组)及空白对照组4组。应用MTT法筛选5-FU作用于人子宫颈腺癌HeLa细胞的药物浓度(48hIC50);经AnnexinⅤ-FITC/PI双标记流式细胞技术(FCM)进行细胞凋亡分析;用荧光显微镜观察各组细胞经作用后凋亡的形态学变化。结果单纯化疗、单纯放疗及放化疗联合都可诱导HeLa细胞凋亡,放化疗联合组与单纯化疗和单纯放疗组比较可明显增加HeLa细胞凋亡率(P<0.05)。结论5-FU和放射线联合应用可明显增加人子宫颈腺癌HeLa细胞的凋亡,为临床应用提供理论依据。Objective To investigate the effect of 5-fluorouracil（5-FU）combined with radiotherapy（RT）on the apoptosis of human cervical cancer HeLa cells. Methods The HeLa cells were divided into four groups treated with chemotherapy（ChT）,RT,RT＋ChT,and control groups. The non-cytotoxic concentration （48 h IC50） of HeLa cells treated with 5-FU was determined by methyl thiazolyl tetrazolium （MTT）and the apoptosis rates of HeLa cells detected by flow cytometry with annexin Ⅴ-FITC and PI double labeling. The morphologic changes of the apoptosis cells were observed under fluorescence microsope. Results ChT,RT and RT＋ChT treatment could induce the apoptosis of HeLa cells with the strong induction of the combined treatment （P 〈 0.05）. Conclusion 5-FU combined with RT can obviously enhance the apoptosis of HeLa cells,which provides a reliable experimental evidence for clinical use of 5-FU combined with RT in the intervention of human cervical cancer.

关 键 词：5-氟尿嘧啶 放射线 HELA细胞 凋亡 

分 类 号：R711.74[医药卫生—妇产科学]