煤焦油对小鼠NIH/3T3细胞间隙连接通讯影响的研究  

Effects of Coal Tar on Gap Junction Intercellular Communication in NIH/3T3 Cells of Mice

作　　者：陈丽丽[1] 王玥[1] 吴永会[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150081

出　　处：《工业卫生与职业病》2010年第1期29-32,共4页Industrial Health and Occupational Diseases

基　　金：哈尔滨医科大学青年科学基金(060005)

摘　　要：目的探讨煤焦油对小鼠肺成纤维细胞(NI H/3T3)的细胞间隙连接通讯(GJIC)功能的影响。方法以小鼠NI H/3T3细胞为靶细胞,煤焦油对NI H/3T3细胞GJIC的影响采用划痕标记染料示踪(SLDT)技术测定。结果煤焦油低、中、高剂量(0.1、1.5、22.5μg/ml)作用6 h后,高剂量组与阴性对照组比较,GJIC的平均水平下降,差异有统计学意义(P<0.05);随着作用时间延长至12 h,各剂量组与阴性对照组比较,GJIC水平再次下降,差异均具有统计学意义(P<0.05),并具有一定剂量-反应关系。结论煤焦油能够抑制GJIC功能。Objective To investigate the effects of coal tar on gap junction intercellular communication （GJIC）in NIH/3T3 cells of mice. Methods Taking NIH/3T3 cells of mice as the target cells, the effects of coal tar on GJIC in NIH/3T3 cells were examined by the scrape-loading and dye transfer（SLDT）technique. Results The NIH/3T3 cells were exposed to coal tar of 0.1, 1.5 and 22.5 μg/ml for 6 hours respectively. Compared with the control group, the level of GJIC in the highest dose group lowered when exposed for 6 hours, the difference was significant （P〈0. 05）. As the exposure prolonged to 12 hours, the levels of GJIC in NIH/3T3 cells in all dosage groups further lowered, all the differences were significant （P〈0. 05） showing dose-response relationship. Conclusions The coal tar might inhibit the GJIC function of NIH/3T3 cells.

关 键 词：细胞间隙连接通讯 NIH/3T3 煤焦油 

分 类 号：R994.3[医药卫生—毒理学]