血清HIV抗体阴性静脉药瘾者肝组织HIVDNA测定  

Detection of HIV DNA by nPCR in liver tissue of anti-HIV seronegative intranous drug users

作　　者：吴南屏[1] 刘克洲[1] 陈智[1] 李继承[1] R.H.DENNIN 

机构地区：[1]浙江医科大学传染病研究所 [2]德国吕贝克医科大学

出　　处：《中华传染病杂志》1998年第4期206-208,共3页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金

摘　　要：目的了解血清抗－ＨＩＶ阴性的静脉药瘾者肝组织是否存在前病毒ＨＩＶＤＮＡ。方法选择３８例抗－ＨＩＶ阴性的静脉药瘾者肝组织，１０例非静脉药瘾者肝组织，用巢式聚合酶链反应，分别在ＨＩＶｇａｇ、ｐｏｌ、ｅｎｖ三大结构区，用六对引物进行ＨＩＶＤＮＡ扩增。结果３８例中有３例同时在三个区域测到ＨＩＶＤＮＡ，１２例分别在二个区域、１７例在单一区域检测到ＨＩＶＤＮＡ，６例在ＨＩＶ三个区域均阴性。对照组１０例无一例检到ＨＩＶＤＮＡ。结论抗－ＨＩＶ阴性的静脉药瘾高危人群，存在潜在ＨＩＶ感染。提示潜在ＨＩＶ感染的检测及加强“窗口期”Objective The study was designed to investigate whether there existed provirus HIV DNA in the liver tissues of intravenous drug users (IVDU) with seronegative results of anti HIV test.Methods We detected 38 liver tissues of seronegative IVDUS and 10 liver tissues of non IVDUS.Using different primers in HIV gag,env as well as pol regions respectively,HIV DNA was detected by nPCR.Results In all 38 cases,3 simultaneously reacted to all three HIV regions (HIV,gag,env,pol),12 reacted to two HIV regions,17 reacted to single HIV region and 6 cases did not react to any HIV region.No sample of control group was found reacting to any HIV region.Conclusion It showed that there might exist HIV infection is some of HIV seronegative IVDUS at high risk of HIV infection.So it is very important to detect potential infection and prevent “windows” HIV infection.

关 键 词：静脉药瘾 NDA 艾滋病毒 抗-HIV 

分 类 号：R512.910.4[医药卫生—内科学] R595.5[医药卫生—临床医学]