HBsAg、HBcAg的免疫荧光检测在HBV-GN诊断中的应用  

Application of HBsAg and HBcAg Immune Fluorescence Detection in Diagnosis of Hepatitis B Virus Associated Glomerulonephritis

作　　者：陈余朋[1] 陈虹[1] 王行富[1] 张声[1] 彭卫华[2] 郑珂[1] 

机构地区：[1]福建医科大学附属第一医院病理科,福建福州350005 [2]南京军区福州总医院肾内科,福建福州350025

出　　处：《中国现代医生》2010年第4期12-13,18,共3页China Modern Doctor

摘　　要：目的观察乙型肝炎病毒相关性肾炎(HBV-GN)患者肾脏组织中HBsAg、HBcAg的表达情况。方法以50例HBV-GN患者为研究对象,另随机选择20例非乙型肝炎病毒相关性肾炎(NHBV-GN)患者作为对照组,用间接免疫荧光法检测肾穿刺活检组织HBsAg、HBcAg的表达。结果HBV-GN患者肾组织中可检测HBsAg、HBcAg阳性颗粒,阳性率分别70%(35/50),24%(12/50);20例(NHBV-GN)患者肾脏组织HBsAg、HBcAg均未检出。与HBV-GN差异有统计学意义(P<0.05)。结论HBsAg、HBcAg免疫荧光标记是诊断HBV-GN的重要指标,HBV在肾组织内存在感染及复制,其在乙型肝炎病毒相关性肾炎的发病机制中具有重要作用。Objective To observe the HBsAg and HBcAg expression in the renal tissue in hepatitis B virus associated glomerulonephritis （HBV-GN）. Methods We selected 50 cases of HBV-GN as research group and the other randomly selected 20 cases of non-HBV-GN（NHBV-GN）as control group and used indirect immunofluorescence assay to detect the HBsAg and HBcAg expression in the renal puncture biopsies. Results The HBsAg and HBcAg-positive particles in the HBV-GN renal tissue could be detected,with the positive rates of 70%（35/50）and 24%（12/50）,while HBsAg and HBcAg failed to be detected in the kidney tissue of 20 cases of NHBV-GN,with a significant difference between the two groups （P〈0.05）. Conclusion The immune fluorescence detection of HBsAg and HBcAg is an important indicator in the diagnosis of HBV-GN. HBV plays an important role in the kidney tissue infection and replication and the pathogenesis of HBV-GN.

关 键 词：HBSAG HBCAG 乙型肝炎病毒相关性肾炎 肾脏 免疫荧光 

分 类 号：R446.8[医药卫生—诊断学]