扁蓿豆不同品系ISSR标记遗传差异和遗传多样性  

Genetic Difference and Genetic Diversity Analysis of Melilotoides ruthenica Strains Based on ISSR Markers

作　　者：王照兰[1,2] 杨持[1] 赵丽丽[3] 胡卉芳[2] 杜建材[2] 白美琴[4] 

机构地区：[1]内蒙古大学生命科学学院,内蒙古呼和浩特010021 [2]中国农业科学院草原研究所/农业部草原资源与生态重点实验室,内蒙古呼和浩特010010 [3]贵州大学,贵州贵阳550025 [4]鄂尔多斯市国土资源局土地勘测规划院,内蒙古东胜017000

出　　处：《中国草地学报》2010年第1期11-17,共7页Chinese Journal of Grassland

基　　金：中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)资助项目;牧草育种技术研究及产业开发项目(2008BADB3B02;2008BADB3B06);国家重点基础研究发展计划(2007CB106800);内蒙古大学博士后资助项目(204102);农业部草原资源与生态重点开放实验室资助

摘　　要：采用ISSR分子标记技术对扁蓿豆4个品系的遗传多样性、遗传差异和遗传结构进行了研究。用7个有效引物对4个品系进行PCR扩增,共获得73个等位基因位点,每条引物平均检测出10.4个位点,其中多态性位点69个,多态位点百分率为94.5%。各品系的Nei’s遗传多样性为0.2569,Shannon’s信息指数为0.4046。Nei’s指数估算和分子方差分析均表明,扁蓿豆4个品系的遗传多样性主要存在于品系内,4个品系间亦出现了较大的遗传分化(Gst=0.1324)。品系00-61、90-36、00-81和93-21的多态位点百分率分别为73.97%、79.45%、82.19%和83.56%;Nei’s遗传多样性(H)分别为0.2050、0.2153、0.2264和0.2474;Shannon’s信息指数(I)分别为0.3222、0.3396、0.3538和0.3821。各品系的遗传参数大小排序均为品系93-21>品系00-81>品系90-36>品系00-61。采用系统聚类和主坐标分析(PCA)两种方法所获得的聚类结果一致,即品系00-61和00-81遗传差异相对较小,其次是品系90-36,品系93-21与各品系的遗传差异较大。The genetic diversity,genetic difference and genetic structure of four strains of Melilotoides ruthenica were investigated with ISSR marker.Seven ISSR primers generated highly reproducible and stable DNA fragments.69 of 73 loci were polymorphic（PPB=94.5%）.The results of Popgen32 analysis indicated that Nei＇s gene diversity（H） was 0.2569,Shannon information index（I） was 0.4046.Nei＇s analysis and AMOVA analysis both indicated that the majority genetic variation of M.ruthenica strains occurred within strains,higher variations also occurred among the strains（Gst=0.1324）.The PPB of strains 00-61,90-36,00-81 and 93-21 was 73.97%,79.45%,82.19% and 83.56%,respectively;Nei＇s gene diversity（H） was 0.2050,0.2153,0.2264 and 0.2474,respectively;Shannon information index（I） was 0.3222,0.3396,0.3538 and 0.3821,respectively.The sequence of all genetic index above was 93-21〉00-81〉90-36〉00-61.The results of cluster analysis and principal coordinate analysis（PCA） were similar.The genetic difference between strain 00-61 and 00-81 was relatively lower,second was strain 90-36,and strain 93-21 had higher genetic difference from other strains.

关 键 词：扁蓿豆 品系 ISSR 遗传差异 遗传多样性 

分 类 号：S541.024[农业科学—作物学]