VEGF、bFGF低频超声透皮给药系统对猪超长筋膜皮瓣存活的影响  

The Application of VEGF and bFGF Via Low-Frequency Ultrasound-Mediated Transdermal Drug Transport System in the Improvement of Maximizing Survival of Fascio-Cutaneous flap with Super Length Ratio in Pigs

作　　者：吕川[1] 陶然[1] 张敬德[1] 宋建星[1] 王晓云[1] 杨超[1] 

机构地区：[1]上海第二军医大学附属长海医院整形外科,上海市200433

出　　处：《组织工程与重建外科杂志》2010年第1期21-23,共3页Journal of Tissue Engineering and Reconstructive Surgery

摘　　要：目的应用VEGF和bFGF,以透皮低频超声导入的无创给药方法,比较单一药物和联合用药对猪超长宽比例筋膜皮瓣成活率及其微循环的影响,为扩大超长宽比例筋膜皮瓣的临床应用奠定实验基础。方法选用10只猪为实验动物。在猪背部两侧设计3 cm×15 cm的筋膜皮瓣,分为空白超声对照组、超声导入VEGF组、超声导入bFGF组、注射VEGF+bFGF联用组、超声导入VEGF+bFGF联用组等5组。术后10 d,测量皮瓣成活面积,测定毛细血管密度以及表达指数,并进行定量分析。结果超声导入VEGF+bFGF联用组皮瓣成活率、毛细血管密度及表达指数等,均明显优于其他4组,差异有显著性(P<0.01)。结论VEGF和bFGF经皮联合低频超声介导给药应用于猪超长宽比例筋膜皮瓣,可显著提高皮瓣的成活率。Objective To explore the effects of single or combined application of VEGF and bFGF via the noninvasive medication of ultrasound-mediated transdermal drug transport （UMTDT） in the survival rate and microcirculation of fasciocutaneous flap with big ratio of length to width. Methods A total of 100 fascio-cutaneous flaps were performed on the sides of the back in ten pigs. The flap area ranged from 3 cm × 15 cm. These flaps were randomly divided into 5 groups as follows. A： control group with blank ultrasound; B： VEGF by low-frequency UMTDT; C： bFGF by low-frequency UMTDT; D： VEGF and bFGF by localized injection in multiple points; E： VEGF and bFGF by low-frequency UMTDT. The quantitative analysis of the vitality of the flaps, capillary intensity and expression index were observed 10 days after operation. Results There was significant difference between the group of combined medication of both and bFGF via UMTDT and the other four groups in flap survival rate, capillary intensity and expression index. Conclusion The combined medication of VEGF and bFGF via UMTDT can significantly enhance the survival rate of fascio-cutaneous flaps with big ratio of length to width.

关 键 词：血管内皮生长因子 碱性成纤维生长因子 低频超声 经皮给药 超长皮瓣 筋膜皮瓣 

分 类 号：R622.9[医药卫生—整形外科]