Effects of ytterbium on outward K^+ currents in NIH3T3 cell  

作　　者：ZHANG LiPing CHEN Ming YANG Pin 

机构地区：[1]Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University Taiyuan 030006, China [2]Institute of Environment Science, Taiyuan University of Science and Technology, Taiyuan 030024, China [3]Institute of Life Sciences, Taishan Medical College, Tai'an 271000, China

出　　处：《Chinese Science Bulletin》2010年第3期246-250,共5页

基　　金：supported by the National Natural Science Foundation of China (Grant No. 20637010);Shanxi Provincial Natural Science Foun-dation for Youth (Grant No. 2007021010)

摘　　要：Using the whole cell patch-clamp technique, we observed the outward K+ currents and studied for the first time the effects of Yb3+ on the currents and kinetics of activation and inactivation in non-excitable NIH3T3 cell. Our results show that the outward K+ currents were promoted with increasing concentration of Ca2+ in pipette solution and saturated at the concentration of 100 μmol/L Ca2+. Yb3+ in bath solution inhibited the currents in a concentration-dependent manner. At the concentration of 1 μmol/L, Yb3+ significantly changed the Vh of activation curve and shifted the activation curve to more negative potentials, but with no effect on its slope factor k. Meanwhile, it had no effect on the inactivation curve. Potassium currents inhibition can induce a series of physiological and molecular biological functions, presumably because of its ability to depolarize the plasma membrane and enhance cell excitability resulting in increasing Ca2+ influx and cytoplast Ca2+ concentration. This process may be one of the mo- lecular mechanisms to affect cell division and proliferation of Yb3+ on NIH3T3 cells.Using the whole cell patch-clamp technique, we observed the outward K^＋ currents and studied for the first time the effects of Yb^3＋ on the currents and kinetics of activation and inactivation in non-excitable NIH3T3 cell. Our results show that the outward K^＋ currents were promoted with increasing concentration of Ca^2＋ in pipette solution and saturated at the concentration of 100 μmol/L Ca^2＋. Yb^3＋ in bath solution inhibited the currents in a concentration-dependent manner. At the concentration of 1 μmol/L, Yb^3＋ significantly changed the Vh of activation curve and shifted the activation curve to more negative potentials, but with no effect on its slope factor k. Meanwhile, it had no effect on the inactivation curve. Potassium currents inhibition can induce a series of physiological and molecular biological functions, presumably because of its ability to depolarize the plasma membrane and enhance cell excitability resulting in increasing Ca^2＋ influx and cytoplast Ca＆2＋ concentration. This process may be one of the molecular mechanisms to affect cell division and proliferation of Yb^3＋ on NIH3T3 cells.

关 键 词：NIH3T3细胞 钾电流 全细胞膜片钳技术 镱 饱和浓度 浓度依赖性 生物学功能 胞质钙 

分 类 号：Q782[生物学—分子生物学] Q485