抗Dectin-1单克隆抗体在白念珠菌刺激小鼠腹腔巨噬细胞释放TNF-α和NO中的作用  

The Role of Anti-Dectin-1 Monoclonal Antibody in the Production of TNF-α and NO by Murine Macrophage Stimulated by Candida Albicans

作　　者：许莉[1] 陈宏翔[1] 俞莺[1] 谭明[1] 李娟[1] 涂亚庭[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,湖北武汉430022

出　　处：《中国皮肤性病学杂志》2010年第2期106-108,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的探讨抗Dectin-1单克隆抗体在白念珠菌刺激小鼠腹腔巨噬细胞释放肿瘤坏死因子-α(TNF-α)和一氧化氮(NO)中的作用。方法①体外培养小鼠腹腔巨噬细胞,设置处理组(抗Dectin-1单克隆抗体组)、阴性对照组与空白对照组,分别给予灭活或活的白念珠菌刺激。②刺激1h,3h,6h,8h后用酶联免疫吸附试验检测各组分泌的TNF-α水平,③刺激6h后Griess法检测各组产生NO的水平。结果①刺激1h后抗体浓度为20μg/mL处理组分泌的TNF-α水平(79.82±11.74pg/mL)低于阴性对照组(105.15±12.36pg/mL)(P<0.05),3h,6h,8h后各浓度处理组TNF-α水平均低于阴性对照组(P均<0.01),且呈浓度依赖性;②6h后各浓度处理组NO水平(61.24±8.85μmol/L,45.36±3.92μmol/L,36.37±2.58μmol/L)均低于阴性对照组(87.65±9.17μmol/L)(P<0.05)。结论抗Dectin-1单克隆抗体在白念珠菌刺激小鼠巨噬细胞释放TNF-α和NO中发挥抑制作用。Objective To investigate the role of anti-Dectin-1 monoclonal antibody in the production of tumor necrosis factor-alpha （TNF-α） and Nitric Oxide （NO） by murine macrophage stimulated with Candida albicans. Methods ①Mouse peritoneal macrophages were preincubated with anti-Dectin-1 monoclonal antibodies in different concentrations in disposal groups; while in negative control groups, an isotype-matched control monoclonal antibodies was used. ②TNF-α concentrations in the supernatant of each group were measured by enzyme-linked immunosorbent assay after stimulated with heat-killed or live Candida albicans for 1 h, 3h, 6h, 8h. ③The NO level in the supernatant were determined by Griess reaction after stimulated with heat-killed or live Candida albieans for 6h. Results ①The TNF-α concentration was significant（79.82±11.74μg/mL） lower than that of the negative control group（105.15±12.36pg/mL） after stimulated for I h in the disposal group of 20μg/mL anti-Dectin-1 monoclonal antibodies （P 〈 0.05）. However, after stimulated for 3h, 6h, 8h, the TNF-α concentrations in the supernatant of the disposal groups were much lower than that of the negative control groups（P 〈 0.01 ）. The NO concentrations of the disposal groups （61.24±8.85μmol/L,45.36±3.92μmol/L,36.37±2.58μmol/L） were lower than that of the negative control groups（87.65±9.17μmol/L） after stimulated for 6h （P 〈0.05）. Conclusion Anti-Dectin-1 monoclonal antibody plays an inhibitory role in the production of TNF-α and NO by murine macrophage after stimulated with Candida albicans.

关 键 词：DECTIN-1 细胞培养 白念珠菌 单克隆抗体 巨噬细胞 肿瘤坏死因子-α 一氧化氯 

分 类 号：R392.11[医药卫生—免疫学] R379.4[医药卫生—基础医学]