神经生长因子在特应性皮炎小鼠模型Th1/Th2类细胞因子免疫失衡中的作用  

The Effect of Nerve Growth Factor on Th1/Th2 Cell Immune Imbalance in Atopic Dermatitis Mice Model

作　　者：何荣国[1] 伍绍国[2] 邬运学[1] 武钦学[1] 田华[1] 

机构地区：[1]广州市第十二人民医院皮肤科,广东广州510620 [2]广州市第十二人民医院检验科,广东广州510620

出　　处：《中国皮肤性病学杂志》2010年第2期109-111,共3页The Chinese Journal of Dermatovenereology

基　　金：广东省医学科研项目(A2009572);广州市医药卫生科技项目(2008-YB-117)

摘　　要：目的探讨神经生长因子(NGF)在特应性皮炎小鼠Th1/Th2类细胞因子免疫失衡中的作用。方法将雌性BALB/c小鼠随机分为四组:正常对照组、特应性皮炎组(AD组)、NGF干预组(NGF组)及抗NGF抗体干预组(NGF阻断组),每组6只。用卵清蛋白经皮肤致敏建立小鼠AD模型。ELISA测定血清中总IgE水平,RT-PCR半定量检测皮肤组织中IL-4和IFN-γmRNA的表达。结果NGF处理组中血清总IgE水平和皮肤组织IL-4mRNA表达水平均高于未处理AD组[(7292.154±1352.654)pg/mL和(4782.145±1132.121)pg/mL;(78.50±3.94)%和(60.67±3.56)%](P<0.01),而IFN-γmRNA表达呈现反相变化[(24.33±2.94)%和(43.17±5.46)%](P<0.01)。NGF抗体干预处理,能阻断NGF诱导IgE和IL-4mRNA的表达[(1475.532±512.476)pg/mL;(39.83±2.64)%](P<0.01)。结论NGF通过诱导Th1型向Th2型免疫应答偏移来参与特应性皮炎Th1/Th2类细胞因子免疫失衡效应。Objective To explore the effect of nerve growth factor（ NGF）on Thl/Th2 cell immune imbalance in atopic dermatitis mice. Methods 24 female BALB/c mice were divided into 4 groups ： normal control group , atopic dermatitis （AD） group, NGF group, and anti-NGF-antibody group. The mouse model of atopic dermatitis were established by sensitization with ovalbumin. Serum IgE level was measured by ELISA. Expression of IL-4 and IFN-γmRNA was detected by RT-PCR. Results As compared with AD group, the level of serum total IgE and the expression of IL-4 mRNA in skin tissue were enhanced remarkedly in the NGF group [ （7292.154±1352. 654） pg/mL vs （4782. 145±1132. 121 ） pg/mL ; （ 78.50±3.94 ） % vs （ 60.67±3.56）% ] （P 〈0. 01）, however the expression of IFN-γmRNA was decreased significantly [ （24.33±2.94） % vs （43.17±5.46）% ] （P 〈0.01 ）. When the activity of NGF was blocked by anti-NGF antibody, the levels of serum total IgE and IL-4 mRNA in skin tissue were repressed[ （ 1475. 532±512.476）pg/mL; （39.83±2.64） % ] （P 〈 0.01 ）. Conclusion Through induction of Th1 type to Th2 type immune response deviation, NGF may be involved in Th1/Th2 immune imbalance in the atopic dermatitis.

关 键 词：特应性皮炎 神经生长因子 白细胞介素-4 干扰素-Γ 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]