利福平-异烟肼-控释型载药人工骨的实验研究  

Experimental Study of a Rifampicine-isoniazidcontrolled Release Drug Implant

作　　者：伍卫刚[1] 郑启新[1] 郭晓东[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022

出　　处：《中国生物医学工程学报》2010年第1期137-143,共7页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30973027);"十一五"国家高技术研究发展计划(863)干细胞与组织工程重大项目(2006AA02A124863);教育部"新世纪优秀人才支持计划资助"(NCET-05-0647)

摘　　要：开发研究一种能承载多药并有控释特性的载药人工骨。利用三维打印技术，制备具有多层同心圆柱体结构的载药人工骨，并将利福平和异烟肼由内到外按特定顺序加载，观察微观结构、孔隙率、体外药物释放特性和体外细胞生物相容性。所制备的载药人工骨呈多孔结构，孔隙率（61．76±2．53）％、微孔孔径50—100μm，体外药物呈现序贯释放，双药释放峰值依次交替出现，持续释放时间超过50d，NTT检测示细胞毒性0级，电镜观察细胞生长分化良好，并有大量细胞黏附于载药人工骨表面；三维打印技术可以精确地制备具有复杂结构的载药人工骨，制备的多药控释型载药人工骨具有药物缓释和序贯控释的特性，同时具有良好的孔隙率和细胞相容性，为骨结核治疗提供一种新型有效的手段。This study is aimed to investigate a controlled release multi-drug implant for bone tuberculosis therapy. 3D printing technology was used to fabricate a drug implant with a multi-layered concentric cylinder construction. Rifampicine and isonicotinic acid hydrazide were placed in a special sequence from the center to the periphery simultaneously. The micro-construction, porosity, drug release property and cytocompatibility were observed in vitro. The controlled release multi-drug implant fabricated by 3D printing were completely coincident with the predefined construction. A multi-aperture construction was detected by ESEM with a porosity of （61.76±2.53）% and micropore diameters ranged from 50-100 μm. An orderly drug release from the outside to the center were detected in the drug release assays in vitro and the release remained over 50 days ; The cytotoxic level detected in MTT assay was 0, and BMSCs cell shave dramatically reproduced and almost covered the whole wall of drug implant. 3D printing technology could fabricate a complex construction drug implant precisely. The drug implant with a multi-layered concentric construction possessed potential capabilities in multi-drugs therapeutic alliance, controlled or programmed drug release, and ideal cytocompatibility, which might offer a new and effective method for bone tuberculosis therapy.

关 键 词：快速成型 三维打印技术 载药人工骨 骨结核 

分 类 号：R318[医药卫生—生物医学工程]