胶原/羟基磷灰石骨软骨一体化支架的生物学特性研究  

Biological properties of a collagen/hydroxyapatite integrated composite scaffold used in osteochondral repair

作　　者：杨波[1] 胡蕴玉[1] 毕龙[1] 李丹[1] 刘民[1] 李静[1] 王迎军[2] 吴刚[2] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032 [2]华南理工大学材料科学与工程学院,广州510641

出　　处：《中国矫形外科杂志》2010年第4期304-307,共4页Orthopedic Journal of China

基　　金：国家科技支撑计划项目(编号:NO2006BAI16B04)

摘　　要：[目的]对体外条件下胶原/羟基磷灰石一体化复合材料的生物学特性进行评价,探讨其作为骨软骨组织工程支架的可行性。[方法]以胶原和羟基磷灰石为原料,研制出由胶原逐层过渡到羟基磷灰石为主的一体化支架材料,其软骨层主要成分为胶原,骨层主要成分为羟基磷灰石。通过扫描电镜观察材料内部结构及孔径大小,液体位移法测定材料的孔隙率。将材料与兔骨髓基质细胞复合培养,MTT法测定细胞的生长曲线,扫描电镜观察细胞在材料上的生长粘附情况。[结果]胶原/羟基磷灰石一体化复合支架具有疏松多孔结构,其中软骨层孔径大小(90±15)μm,骨层孔径大小(120±20)μm,孔隙率(75±5.0)%。细胞在材料上生长良好。[结论]胶原/羟基磷灰石一体化复合材料具有适宜的孔隙结构和良好的生物相容性,可用作骨软骨组织工程支架材料。[Objective]To observe the biological properties of a novel collagen/hydroxyapatite composite scaffold in vitro and to evaluate the possibility of application being used in tissue engineering for osteochondral repair.[Method]The scaffolds were constructed of collagen I and hydroxyapatite.The pore size and interpores of the scaffold were observed by scanning electronmicroscopy（SEM）.The porosity was measured by liquid displacement method.Rabbit bone marrow stromal cells（BMSCs） were isolated and amplified,then inoculated onto the scaffold.By SEM scanning,the condition of the cells adhering onto the scaffold was observed.The proliferation of the cells on the scaffolds was examined using MTT method,and the growth curve was drawn.[Result]The scaffold possessed high porosity and proper pore size.The pore diameter of the collagen layer was about 90μm,the pore diameter of the HA layer was about 120μm,and the overall porosity of the composite scaffold was 75%.The proliferation of the cells on the scaffold was good.[Conclusion]The novel collagen/hydroxyapatite composite scaffold possesses desirable pore structure and good biocompatibility,and it can be used in tissue engineering for osteochondral repair.

关 键 词：胶原 羟基磷灰石 骨软骨 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]