化学去细胞异体神经促神经再生的体外实验研究  

Promotion effects of nerve regeneration of chemically extracted acellular nerve allograft in vitro

作　　者：王玉[1] 彭江[1] 张莉[1] 赵斌[1] 赵喆[1] 杨昱[1] 许文静[1] 卢世璧[1] 

机构地区：[1]解放军总医院骨科研究所,北京市复兴路28号100853

出　　处：《中国矫形外科杂志》2010年第4期308-311,共4页Orthopedic Journal of China

基　　金：国家863计划(2009AA03Z312)项目;军队"十一五"计划(06Z057)项目

摘　　要：[目的]对大鼠化学去细胞异体神经蛋白成分进行电泳分析,观察去细胞异体神经中的蛋白组分;制备去细胞神经薄膜,接种背根神经节,观察其结构对神经生长的影响。[方法]按Sondell法制备大鼠的去细胞异体神经,将制备好的神经进行电泳分析,观察28～30 kDa区域的髓鞘蛋白的去除程度;将制备的神经进行冰冻切片,接种鸡胚背根神经,进行神经纤维荧光染色,观察神经纤维在神经切片上的生长方向。[结果]去细胞异体神经电泳结果显示:28～30 kDa区域髓鞘蛋白完全消失,化学萃取的去细胞神经可以完全去除髓鞘蛋白;背根神经节发出大量的神经纤维沿着去细胞神经基底膜管方向生长。[结论]去细胞异体神经中无残留引起免疫反应的髓鞘蛋白,保留的基底膜管结构对促神经纤维再生具有引导性作用。[Objective]Electrophoretic analysis of rat chemically extracted acellular nerve allograft（CEAN） protein was performed to investigate the protein of CEAN.Dorsal root ganglia of chicken were cultured onto the surface of CEAN slice to observe the effect of CEAN structure on the fiber regeneration.[Method]The CEAN were perpared according to Sondell method and electrophoresed to observed whether the electrophoresis strip of 28-30kDa（Myelin Protein） exited or not.Dorsal root ganglia of chicken were cultured onto thc surface of CEAN slice and dyed with nerve fiber fluorescence to observe the orientation of nerve fiber growth on the surface of CEAN slice.[Result]Electrophoretic analysis of rat CEAN protein showed that the electrophoresis strip of 28-30 kDa totally disappeared.A large number of nerve fibers grew from DRG along the basement membrane of CEAN.[Conclusion]No myelin protein remained in the CEAN,while the basement membrane structure of CEAN can induce the nerve fiber to grow.

关 键 词：化学去细胞异体神经 蛋白电泳 背根神经节 神经再生 

分 类 号：R651.3[医药卫生—外科学]