Apoptotic mechanism of MCF-7 breast cells in vivo and in vitro induced by photodynamic therapy with C-phycocyanin  

作　　者：Bing Li Xianming Chu Meihua Gao Wuxiu Li 

机构地区：[1]Department of Biology, Medical College of Qingdao University, Qingdao 266021, China [2]Deparment of Cardiology, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266000, China [3]Shandong University of Science and Technology, Qingdao 265102, China

出　　处：《Acta Biochimica et Biophysica Sinica》2010年第1期80-89,共10页生物化学与生物物理学报（英文版）

摘　　要：The aim of this study was to investigate the pro-apoptotic mechanism of C-phycocyanin （C-PC）-mediated photody- namic therapy （PDT） in a murine tumor model and cul- tured MCF-7 cells. The mice were divided into four groups： control, He-Ne laser radiation, C-PC treatment, and C-PC treatment ＋ He-Ne laser radiation. The effects of C-PC and/or laser on immune organs, immunocyte proliferation, tumor genesis, and apoptosis-related pro- teins expressions were investigated by immunohistochem- istry, in situ hybridization, MTT, electron microscope, western blot, and immunofluorescence assay. The results showed that He-Ne laser treatment alone showed mar- ginal effects. In C-PC-treated mice, the weight of immune organs, proliferation of immunocytes, and expression of pro-apoptotic Fas protein were increased, whereas the tumor weight and the expressions of anti-apoptotic pro- teins （NF-KB and P53） and CD44 mRNA were compara- tively decreased. In vitro, C-PC was able to inhibit MCF-7 cell proliferation and cause ultrastrnctural changes including microvilli loss, formation of membrane blebs, and chromatin condensation. Moreover, C-PC treatment could activate caspase-9 expression, induce cytochrome c release, and downregulate Bel-2 expression. When com- bined with He-Ne laser irradiation, the effects of C-PC treatment were further enhanced. Facilitating the apoptosis signals transduction and finally leading to the apoptosis of MCF-7 cells may be the mechanism of the anti-tumor activities of C-PC-mediated PDT.The aim of this study was to investigate the pro-apoptotic mechanism of C-phycocyanin （C-PC）-mediated photody- namic therapy （PDT） in a murine tumor model and cul- tured MCF-7 cells. The mice were divided into four groups： control, He-Ne laser radiation, C-PC treatment, and C-PC treatment ＋ He-Ne laser radiation. The effects of C-PC and/or laser on immune organs, immunocyte proliferation, tumor genesis, and apoptosis-related pro- teins expressions were investigated by immunohistochem- istry, in situ hybridization, MTT, electron microscope, western blot, and immunofluorescence assay. The results showed that He-Ne laser treatment alone showed mar- ginal effects. In C-PC-treated mice, the weight of immune organs, proliferation of immunocytes, and expression of pro-apoptotic Fas protein were increased, whereas the tumor weight and the expressions of anti-apoptotic pro- teins （NF-KB and P53） and CD44 mRNA were compara- tively decreased. In vitro, C-PC was able to inhibit MCF-7 cell proliferation and cause ultrastrnctural changes including microvilli loss, formation of membrane blebs, and chromatin condensation. Moreover, C-PC treatment could activate caspase-9 expression, induce cytochrome c release, and downregulate Bel-2 expression. When com- bined with He-Ne laser irradiation, the effects of C-PC treatment were further enhanced. Facilitating the apoptosis signals transduction and finally leading to the apoptosis of MCF-7 cells may be the mechanism of the anti-tumor activities of C-PC-mediated PDT.

关 键 词：apoptosis C-PHYCOCYANIN MCF-7 cells He-Ne laser PDT anti-tumor effect 

分 类 号：Q279[生物学—细胞生物学] Q255