人工合成相关寡肽抑制流产发生的免疫机制研究  

Prevention of the I:C-induced abortion with the HCG-derived oligopeptide MTRV

作　　者：王丽丽[1] 曾山孙[1] Nisar Khan 林羿[2] Robbert Benner 芦春斌[1] 

机构地区：[1]暨南大学生殖免疫研究所,广州510632 [2]暨南大学组织移植与免疫研究中心,广州510632 [3]荷兰鹿特丹Erasmus医学中心,荷兰

出　　处：《现代免疫学》2010年第1期14-18,共5页Current Immunology

基　　金：国家自然科学基金资助项目(30672231、30730087)

摘　　要：研究寡肽蛋-苏-精-缬(MTRV)抑制多聚次黄苷酸-胞苷酸(poly I∶C)诱发的小鼠胚胎吸收的免疫机制。给予异基因妊娠BALB/c×C57BL/6小鼠腹腔注射poly I∶C建立诱发性胚胎吸收模型;在孕期多个时点腹腔注射MTRV,观察其对胚胎吸收率的影响,并采用流式细胞术检测妊娠9.5 d和13.5 d妊娠子宫内各种免疫活性细胞的百分率。结果显示,MTRV不能显著改变poly I∶C所引起的Toll样受体3阳性细胞和CD80阳性细胞增多,但是可显著抑制poly I∶C所引起的IFN-γ阳性细胞增多和转化生长因子-β阳性细胞减少,并降低流产率。提示寡肽MTRV可纠正poly I∶C所引起的妊娠子宫内细胞因子表达紊乱,从而抑制poly I∶C所诱发的流产。To determine whether the HCG-derived oligopeptide MTRV（Met-Arg-Val） can prevent the poly I∶C-induced embryo-Absorption and to experpre its possible immunological mechanisms,induced embryo-Absorption model was established by peritoneal injection of poly I∶C to female mice of the BALB/c×C578BL/6 mating combination.The administration of MTRV was performed at time points during pregnancy in the poly（I∶C）-induced female mice and the rate of fetal Absorption was measured on day 9.5 and day 13.5 of gestation.Meanwhile,the percentage of TLR3＋,CD80＋,IFN-γ＋,and TGF-β＋ cells among CD45＋ cells was tested in uterine immune cells using flow cytometry.It showed that the multiple administrations of MTRV could not change poly I∶C-induced increase of the relative number of TLR3＋ and CD80＋ cells.However,it could abrogate the poly I∶C-induced increase of IFN-γ＋ cells and the decrease of TGF-β＋ cell number among CD45＋ cells,and inhibit poly I∶C-induced increase of embryo-Absorption.It suggests that hCG-derived oligopeptide MTRV may modulate the functional status of cytokine production at the feto-maternal interface and subsequently inhibit the poly I∶C-induced embryo-Absorption.

关 键 词：流产 母-胎界面 免疫调节 免疫耐受 

分 类 号：R392.11[医药卫生—免疫学]