PGE_2促进人脐血CD34^+造血祖细胞向淋巴样树突状细胞分化  

PGE_2 promotes plasmacytoid dendritic cells development from CD34^+ hematopoietic progenitor cells of human cord blood

作　　者：侯静[1] 李明峰[1] 熊伟民[1] 顾剑锋[1] 陈兆远[1] 张雁云[2] 

机构地区：[1]中国人民解放军第四五五医院消化肿瘤科,上海200052 [2]中国科学院上海生命科学研究院健康科学研究所,上海200025

出　　处：《现代免疫学》2010年第1期41-45,共5页Current Immunology

基　　金：上海市自然科学基金资助项目(06ZR14117);南京军区卫生科研基金资助项目(06MA40)

摘　　要：为了探讨前列腺素E2(protaglandin E2,PGE2)对人外周血CD14+单核细胞和脐血CD34+造血祖细胞(hematopoieticprogenitor cells,HPC)体外诱导pDC分化的影响。分别采用IL-4+GM-CSF+TNF-α和SCF+Flt-3L+GM-CSF+TNF-α诱导人外周血CD14+单核细胞和CD34+HPC向pDC分化,采用流式细胞仪分析细胞表型,并观察PGE2加入培养体系后对pDC分化的影响。结果:以CD34+HPC为来源,比CD14+单核细胞能诱导出更多数量且具有功能的pDC。培养体系中加入PGE2显著增强了CD34+HPC向pDC的分化,而对CD14+单核细胞的分化却无明显促进作用。PGE2可有效促进脐血CD34+HPC在多种细胞因子的作用下向pDC的成熟分化。To investigate the experimental methods of inducing pDC differentiation via CD14＋ peripheral blood mononuclear cells（PBMC） and CD34＋ hematopoietic progenitor cells（HPC） and the effect of protaglandin E2（PGE2） in vitro.Methods： CD14＋ monocytes and CD34＋ HPC were isolated from PBMC and healthy umbilical cord blood respectively.Mature CD14＋ PBMC-derived pDC and DC34＋ HPC-derived pDC were generated with IL-4 ＋ GM-CSF ＋ TNF-α or SCF ＋ Flt-3L ＋ GM-CSF ＋ TNF-α with or without PGE2.Then the membrane surface markers of pDC were identified by FCM.Results： CD34＋ HPC induced more functional pDC than CD14＋ PBMC in certain culture systems.And PGE2 largely enhanced the DC34＋ HPC-derived pDC differentiation,while did not have significant effect to the pDC differentiation induced by CD14＋ PBMC.Conclusions： PGE2 can effectively improve the mature differentiation of pDC with other cytokines.

关 键 词：淋巴样树突状细胞 前列腺素E2 CD14+单核细胞 CD34+造血祖细胞 

分 类 号：R392.12[医药卫生—免疫学]