内皮祖细胞移植对兔心肌梗死后心功能的影响  

Effect of endothelial progenitor cell transplantation on cardiac function in rabbits with myocardial infarction

作　　者：许爱斌[1] 宋延彬[2] 郭文怡[1] 李飞[1] 王海昌[1] 谢晓莉[1] 

机构地区：[1]第四军医大学西京医院心内科,陕西西安710032 [2]延安大学附属医院心内科,陕西延安716000

出　　处：《心脏杂志》2010年第1期56-58,共3页Chinese Heart Journal

摘　　要：目的:探讨移植内皮祖细胞(EPCs)对兔心肌梗死后心功能的影响。方法:通过密度梯度离心法体外分离兔单个核细胞,接种于人纤连蛋白包被的培养板上,并给予含血管内皮生长因子(VEGF)20μg/L的EBM-2完全培养液培养2周。取健康大耳白兔,结扎左冠状动脉前降支,并将制作心肌梗死模型成功的大耳白兔随机分为两组,即对照组和实验组,每组20只。2周后实验组心外膜下直接注射EPCs(1.6×1010/L)悬液,对照组注射无菌PBS液。此外,两组均给予心肌梗死后常规药物治疗。术后4周,分别行超声心动图检查和血浆脑钠肽(BNP)浓度测定及计数移植区毛细血管。结果:实验组左室舒张末期内径(LVEDD)与术前及对照组相比均无统计学差异,而实验组左室射血分数(LVEF)与术前及对照组相比有统计学差异(P<0.05)。实验组血浆BNP水平较术前降低,差异有统计学意义(P<0.01),且与对照组相比,血浆BNP水平明显降低(P<0.05)。移植细胞4周后,实验组梗死边缘区毛细血管密度与对照组有显著性差异(P<0.05)。结论:心外膜下直接注射移植EPCs能够改善兔心肌梗死后的心功能,延缓慢性心力衰竭的发生和发展。AIM： To explore the effect of endothelial progenitor cell transplantation on cardiac function in rabbits with myocardial infarction. METHODS： Forty rabbits were randomly divided into two groups： experiment group and control group. After two weeks, myocardial infarction was induced by ligating the left anterior descending branch of the coronary artery and both groups received standard drug therapy. Four weeks after transplantation, changes in cardiac function were examined by echocardiography and brain natriuretic peptide （BNP） levels. RESULTS： Left ventricular ejection fraction （LVEF） and sizes of LVEDD in post-transplantation group were not significantly different from those pre-transplantation and in control group. Plasma BNP level decreased significantly at 4 weeks after endothelial progenitor cell transplantation compared with pre-transplantation and in control group （ P 〈 0.05 ）. CONCLUSION： Endothelial progenitor cell transplantation improves cardiac function after myocardial infarction.

关 键 词：内皮祖细胞 心肌梗死 心功能 兔 

分 类 号：R542.2[医药卫生—心血管疾病]