麻竹花药培养及再生植株的获得  

Anther Culture and Plant Regeneration of Dendrocalamus latiflorus

作　　者：乔桂荣[1,2] 李海营[1] 蒋晶[1] 孙宗修[2] 卓仁英[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,富阳311400 [2]中国水稻研究所,杭州310006

出　　处：《植物学报》2010年第1期88-90,共3页Chinese Bulletin of Botany

摘　　要：以麻竹(Dendroc alamus latiflorus Munro)花药为材料,于M8+2mg·L-1NAA+0.5mg·L-16-BA+15mg·L-1PAA+7.5mg·L-1STS+500mg·L-1CH+100mg·L-1proline+100mg·L-1glutamin+5.4%maltose+0.8%agar的诱导培养基上成功诱导出胚性愈伤组织,在此培养基上继代可形成体胚并分化成苗,初步建立了麻竹花药一步成苗的再生体系。Embryogenic callus was initiated from bamboo （Dendrocalamus latiflorus Munro） anthers cultured on M8 medium supplemented with 2 mg·L^–1 NAA, 0.5 mg·L^–1 6-BA, 15 mg·L^–1 phenylacetic acid （PAA）, 7.5 mg·L^–1 silver thiosulfate （STS）, 500 mg·L^–1 casein enzymatic hydrolysate （CH）, 100 mg·L^–1 proline, 100 mg·L^–1 glutamine, 5.4% maltose, and 0.8% agar. Subculture of these embryogenic calli on the same medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. A one-step method for anther culture and plant regeneration of D. latiflorus was preliminarily established.

关 键 词：花药培养 麻竹 苯乙酸 

分 类 号：S795[农业科学—林木遗传育种]