原代培养的人网膜前脂肪细胞分化过程中基因表达和脂肪因子分泌的特征  

Characterization of gene expression and adipokine secretion during differentiation of primary cultured human omental preadipocytes

作　　者：黎明[1] 阴津华[2] 张葵[1] 吴从愿[1] 邓洁英[1] 

机构地区：[1]北京协和医院内分泌科,100730 [2]山西医科大学第一医院内分泌科

出　　处：《中华内分泌代谢杂志》2010年第1期56-59,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：基金项目：北京市科技新星计划（2004A27）,国家自然科学基金资助项目（30671804）

摘　　要：采用胶原酶消化法分离原代培养人网膜基质前脂肪细胞进行诱导分化。在诱导分化早期，前脂肪细胞因子1表达迅速降低，而脂蛋白脂酶、PPARγ／2表达迅速增加，CAAT／增强子结合蛋白α（c／EBPα）在成熟阶段表达丰度最高。瘦素mRNA表达和分泌随分化逐渐增高，抵抗素mRNA仅在前脂肪细胞表达和分泌而脂联素mRNA仅在成熟脂肪细胞表达和分泌，胰岛素样生长因子I（IGF-I）在前脂肪细胞诱导分化前后均有分泌。Stromal preadipocytes from human omental adipose tissues were isolated with enzyme-digesting method,and cultured for inducing differentiation. Preadipoeyte faetor-1 was abundant during preadipocyte stage, whereas lipoprotein lipase and PPAR-α2 mRNA were rapidly up-regulated at early stage of differentiation and CCAAT/enhancer binding protein expression was increased rather late. In addition, resistin expression and secretion was primarily detectable in preadipoeytes, leptin was gradually increased during adipogenesis, whereas adiponectin appeared specifically in mature adipocytes. Insulin-like growth factor-I secretion was remarkably detected in both preadipoeytes and mature adipocytes.

关 键 词：网膜脂肪组织 前脂肪细胞 原代培养 脂肪因子 

分 类 号：R346[医药卫生—基础医学]