甘蓝型油菜显性核不育纯合两型系“HY15AB”的选育  

Breeding of homozygous two-type dominant genic male sterile line "HY15AB" of Brassica napus L.

作　　者：周熙荣[1] 庄静[1] 孙超才[1] 王伟荣[1] 李延莉[1] 杨立勇[1] 蒋美艳[1] 顾龙弟[1] 钱小芳[1] 

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201106

出　　处：《上海农业学报》2010年第1期1-4,共4页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2008)第7-3号]资助

摘　　要：以甘蓝型油菜双低品种"沪油15"为轮回亲本,分别以显性核不育性的Ms不育基因和Rf恢复基因为目标基因,通过杂交和回交,将目标基因导入双低品种"沪油15"。通过复交将Ms不育基因和Rf恢复基因融入同一群体,然后采用自交、兄妹交和测交等育种手段,获得育性分离比例为3∶1的自交后代,其基因型为1MsMsrfrf∶2MsMsRfrf∶1MsMsRfRf,最后从该群体配置兄妹交组合,育性分离比例符合1∶1的兄妹交后代就是经回交转育的双低纯合两型系"HY15AB",其基因型分别为MsMsRfrf和MsMsrfrf。Two target genes, a dominant genic male sterility gene （Ms） and a restoring gene （R f）, were transferred respectively to the double-low-content rapeseed cultivar ＂Huyou i5＂ by crossing and backcrossing,and then the two lines were crossed so as to blend Ms gene with Rf gene. By means of selfing,sib mating and test cross, the selfed progeny with the genotype of 1 MsMsrfrf： 2 MsMsRfrf ： 1 MsMsRfRf was obtained,whose fertility segregation ratio was 3 ： 1. At last by sib cross combination a homozygous two-type sterile line ＂HY15AB＂ including MsMsRfrf and MsMsrfrf two genotypes was obtained,and its sib cross always showed a fertility segregation ratio of 1：1.

关 键 词：甘蓝型油菜 显性核不育 纯合两型系 恢复系 

分 类 号：S565.403[农业科学—作物学]