紫杉醇在猪体外成熟卵母细胞玻璃化冷冻中的作用探讨  

Effect of taxol on porcine oocytes of in vitro maturation in vitrification

作　　者：王磊[1,2,3] 杨山亭[1,2,3] 戴建军[1,2] 吴彩凤[1,2] 张廷宇[1,2] 张德福[1,2] 

机构地区：[1]上海市农业科学院畜牧兽医研究所 [2]上海市农业遗传育种重点实验室动物遗传工程研究室,上海201106 [3]南京农业大学动物科技学院,南京210095

出　　处：《上海农业学报》2010年第1期20-23,共4页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2005)第3-5号];上海市科技兴农推广项目(2007-3-7);上海市农业科学院青年科技发展基金[2008(07);2009(15)];上海市农业遗传育种重点实验室开放课题(Shagb2008-03);转基因生物新品种培育重大专项(2009ZX08006-014B;2008ZX08006-005)资助

摘　　要：使用1.0μmol/L紫杉醇预处理卵母细胞,冷冻后其形态完整率(89.93%)和FDA染色存活率(83.33%)均显著高于未处理组的79.12%和70.97%。不同预处理时间试验表明:预处理30min组冷冻后卵母细胞形态完整率和FDA染色存活率最高,分别达到90.21%和84.13%。预处理浓度1.0μmol/L,30min是比较合适的处理方法;紫杉醇、细胞松弛素B前处理能显著提高猪成熟卵母细胞的玻璃化冷冻的效果,但两者之间没有显著差异。The experiments with M 11 porcine oocytes were carried out with a cryoloop in order to study the effects of taxol concentration and treatment time on vitrification of the porcine oocytes. The results showed that the normal morphology rate（89.93%）of oocytes pretreated with 1.0μmol/L taxol after vitrification and their survival rate（83.33%）after FDA staining were both significantly higher than those of control oocytes（79.12% and 70.97% ）;and when the pretreating time was 30 min,the normal morphology rate after vitrification and survival rate after FDA staining reached the highest, respectively 90.21% and 84.13%. So it was appropriate to pretreat the oocytes with 1.0μmol/L taxol for 30 min. The pretreatment with taxol or cytochalasin B（CB）could significantly improve the results in vitrification of mature porcine oocytes,and both effects of taxol and CB were not significantly different.

关 键 词：猪 卵母细胞 玻璃化冷冻 紫杉醇 

分 类 号：S814.8[农业科学—畜牧学] S828[农业科学—畜牧兽医]