自然流产患者绒毛EVTs的基因芯片初步分析  

Expression profile analysis of human spontaneous abortion extravillous trophoblast cells

作　　者：余秋波[1] 胡建刚[2] 王应雄[2] 黎刚[1] 谭彬[2] 何俊琳[2] 

机构地区：[1]重庆医科大学公共卫生学院生殖毒理教研室 [2]重庆医科大学公共卫生学院遗传优生教研室,重庆400016

出　　处：《重庆医科大学学报》2010年第1期18-22,共5页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:30500054)

摘　　要：目的:探讨自然流产绒毛外滋养细胞(Extravillous trophoblast cells,EVTs)侵袭相关基因表达变化。方法:利用U133plus2.0芯片对自然流产绒毛和正常绒毛组织中EVTs的基因表达谱进行检测,并用生物信息学方法对检测结果进行分析。结果:与正常EVTs相比,在自然流产EVTs中,发现显著上调基因219个,下调基因293个,其功能主要涉及细胞粘附、免疫应答、代谢等。结论:与滋养层细胞细胞粘附、免疫应答、水解酶及凋亡等相关基因的差异表达可能与滋养细胞侵袭行为变化及自然流产有关。Objective： To investigate the expression changes of invasion-relevant genes in spontaneous abortion Extravillous trophoblast cells （EVTs）. Methods： The U133 plus2.0 gene chips were used to analyze gene expression profiles in spontaneous EVTs and normal EVTs.The results were analyzed by bioinformatics methods. Results：Compared with normal EVTs, 219 and 293 genes in spontaneous abortion EVTs were up-regulated and down-regulated respectively. These genes were mainly associated with signal transduction, apoptosis,metabolism,immune response, etc. Conclusion： Differential expression of genes related with apoptosis,immune response, hydratase, cell adhesion may play a role in the EVTs＇ abnormal invasion and spontaneous abortion.

关 键 词：自然流产 EVTs 侵袭 基因表达谱 

分 类 号：R714.21[医药卫生—妇产科学]