番茄子叶不定芽分化的初探  

The Investigation of The Shoot Regeneration of Tomato Cotyledon

作　　者：赵歌[1] 王谨[2] 储荣华[1] 陆小平[1] 

机构地区：[1]苏州大学金螳螂建筑与城市环境学院,江苏苏州215123 [2]苏州工业园区白塘植物生态园,江苏苏州215123

出　　处：《北方园艺》2010年第4期150-153,共4页Northern Horticulture

基　　金：苏州市科技支撑(农业)资助项目(SNG0908)

摘　　要：对番茄子叶不定芽分化进行了探讨。结果表明:在番茄子叶(或子叶切段)离体培养中,以2/3子叶片处切取子叶所获得的外植体,用于子叶不定芽分化的起始材料最好;利用番茄种子的胚体直接进行组培,再从胚体上切取子叶作为外植体进一步培养可有效缩短组织培养周期。The shoot regeneration of tomato cotyledon was studied.The result indicated that： 2/3-cut cotyledon was the best recipient for tomato tissue culture among various explants, including petiole-cotyledon and multiculting cotyledon. The embryo of tomato was cultured in the MS agar medium, then the explants which were cutted from those culturedembryos were planted for tissue culture. By this way, we can shorten the tissue period for tomato culture, and the more vigorous plants can be obtained.

关 键 词：番茄子叶 组织培养 定芽分化 

分 类 号：S641.203.6[农业科学—蔬菜学]