木兰科濒危植物大果木莲遗传多样性的ISSR分析  

Genetic diversity of endangered species Manglietia grandis detected by inter-simple sequence repeat markers

作　　者：陈少瑜[1] 韩燕[2] 吴涛[1] 付玉嫔[1] 司马永康[1] 郝佳波[1] 

机构地区：[1]云南省林业科学院,云南省森林植物培育与开发利用重点实验室,云南昆明650204 [2]西南林学院资源学院,云南昆明650224

出　　处：《福建林学院学报》2010年第1期56-60,共5页Journal of Fujian College of Forestry

基　　金：云南省自然科学基金资助项目(2006C0066M)

摘　　要：采用ISSR分子标记技术对云南省3个大果木莲天然居群进行遗传多样性分析。结果表明,在物种水平上,大果木莲的多态位点百分率(PPB)为70.71%;有效等位基因数(Ne)为1.4121;Nei′s基因多样性指数(H)为0.2433;Shannon信息指数(I)为0.3651。在居群水平上,其PPB为44.1%;Ne为1.2704;H为0.1573;I为0.2343。通过Nei′s遗传多样性分析得到居群间的遗传分化系数(Gst)为0.3595,由遗传一致度进行了3个居群的UPGMA聚类。Inter-simple sequence repeat （ISSR） technique was used to detect 3 natural populations of Manglietia grandis distributed in Yunnan.The results showed that at species level,the value of the average percentage of polymorphic bands （PPB） was 70.71%,effective number of alleles per locus （Ne） was 1.412 1,Nei＇s gene diversity index （H） was 0.243 3 and Shannon＇s information index （I） was 0.365 1.While at population level,the values of PPB,Ne,H and I were 44.1%,1.270 4,0.157 3 and 0.234 3 respectively.The coefficient of population differentiation （Gst） among populations based on Nei＇s genetic distance was 0.359 5.Based on genetic identity,dendrogram of 3 populations was constructed by UPGMA.

关 键 词：大果木莲 濒危植物 ISSR分子标记 遗传多样性 

分 类 号：Q311[生物学—遗传学]