Genome Analysis of the Bombyx mori Infectious Flacherie Virus Isolated in China  

作　　者：LI Ming-qian CHEN Xiao-xue WU Xiao-xia MAN Na-na JIN Wei LU Xing-meng 

机构地区：[1]Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, P.R.China

出　　处：《Agricultural Sciences in China》2010年第2期299-305,共7页中国农业科学（英文版）

基　　金：supported by the National 863 Program of China (2006AA10A119);the Key Project of Science and Technology Commission of Zhejiang Province,China (2003C22013)

摘　　要：We reported here the complete genome of the Bombyx mori infectious flacherie virus （BmlFV）, BmlFV-CHN01, isolated in China and compared it with the Japanese strain IFV （BmIFV-JAN） sequence. BmIFV-JAN and BmIFV-CHN01 were 99% identical in nucleotide and amino acid sequences. The amino acid sequences of the three structural proteins （VP1, VP3 and VP4） and L protein were 100% identical between the two strains. Phylogenetic analyses based on conserved domains in RNA-dependent RNA polymerases （RdRps） by the neighbor-joining method showed that these two strains were from the same virus. The gain of the whole genome of the BmIFV isolated in China and its weak mutation character established a great foundation to the study of functional genome and the molecular epidemiology of BmIFV.We reported here the complete genome of the Bombyx mori infectious flacherie virus （BmlFV）, BmlFV-CHN01, isolated in China and compared it with the Japanese strain IFV （BmIFV-JAN） sequence. BmIFV-JAN and BmIFV-CHN01 were 99% identical in nucleotide and amino acid sequences. The amino acid sequences of the three structural proteins （VP1, VP3 and VP4） and L protein were 100% identical between the two strains. Phylogenetic analyses based on conserved domains in RNA-dependent RNA polymerases （RdRps） by the neighbor-joining method showed that these two strains were from the same virus. The gain of the whole genome of the BmIFV isolated in China and its weak mutation character established a great foundation to the study of functional genome and the molecular epidemiology of BmIFV.

关 键 词：Bombyx mori infectious flacherie virus complete genome phylogenetic analysis 

分 类 号：S858.315.3[农业科学—临床兽医学] Q78[农业科学—兽医学]