Gene Expression Profiles of Response to Water Stress at the Jointing Stage in Wheat  

作　　者：SHI Jun-feng MAO Xin-guo JING Rui-lian PANG Xiao-bin WANG Yu-guo CHANG Xiao-ping 

机构地区：[1]The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm & Biotechnology Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China [2]Institute of Farm Products Storage, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, P.R.China [3]Agronomy College, Shanxi Agricultural University, Taigu 030801, P.R.China

出　　处：《Agricultural Sciences in China》2010年第3期325-330,共6页中国农业科学（英文版）

基　　金：supported by the National High-TechR&D Program of China (863 Program, 2006AA100201);the National Transgenic Plants Program of China(2008ZX08002-002)

摘　　要：Drought is one of the most adverse environmental factors that impact on plant growth and reduce crop yields. To decipher the molecular mechanism of drought tolerance, we constructed a cDNA library using suppression subtractive hybridization （SSH） and dissected the gene expression profiles in seedling and jointing wheat plants after stress with PEG-6000. A total of 2 046 ESTs from the jointing stage library （J-Lib） were allocated to 961 contigs. Among the ESTs, 265 uni-genes in 12 categories were identified on the basis of sequence similarities and functional classifications. Most were known to be involved in protection, directly or indirectly, against water stress. To determine differences in gene expression profiles for water stress responses at the jointing and seedling stages, data from the J-Lib were compared with those from a 2- leaf seedling library （S-Lib） constructed by the same method. Significant differences were observed between the two libraries for function-known genes; signal transduction genes were far more active at jointing than at the seedling stage.Drought is one of the most adverse environmental factors that impact on plant growth and reduce crop yields. To decipher the molecular mechanism of drought tolerance, we constructed a cDNA library using suppression subtractive hybridization （SSH） and dissected the gene expression profiles in seedling and jointing wheat plants after stress with PEG-6000. A total of 2 046 ESTs from the jointing stage library （J-Lib） were allocated to 961 contigs. Among the ESTs, 265 uni-genes in 12 categories were identified on the basis of sequence similarities and functional classifications. Most were known to be involved in protection, directly or indirectly, against water stress. To determine differences in gene expression profiles for water stress responses at the jointing and seedling stages, data from the J-Lib were compared with those from a 2- leaf seedling library （S-Lib） constructed by the same method. Significant differences were observed between the two libraries for function-known genes; signal transduction genes were far more active at jointing than at the seedling stage.

关 键 词：WHEAT suppression subtractive hybridization library EST drought stress 

分 类 号：S512.101[农业科学—作物学] Q786[生物学—分子生物学]