HPLC-ELSD法测定银杏叶分散片中萜内酯的含量  

Content Determination of Terpene Lactones by HPLC-ELSD in Ginkgo Leaves Dispersible Tablets

作　　者：谷薇薇[1] 张国升[1] 刘德娟[1] 刘丛彬[1] 张福田 

机构地区：[1]安徽中医学院药学院,安徽合肥230031 [2]合肥大众医药科技开发有限公司,安徽合肥230031

出　　处：《安徽中医学院学报》2010年第1期73-75,共3页Journal of Anhui Traditional Chinese Medical College

摘　　要：目的建立银杏叶分散片中萜内酯的含量测定方法。方法采用蒸发光散射检测器，以ODS柱（150mm×4．6mm）为色谱柱，以正丙醇-四氢呋喃-水（1：15：84）为流动相。结果白果内酯在7．848～39．24μg、银杏内酯A在3．8～19．0μg、银杏内酯B在7．08-35．40μg、银杏内酯C在3．768～18．840μg范围内进样量的对数与峰面积的对数线性关系较好，平均回收率依次分别为98．39％（RSD=0．31％）、98．41％（RSD=0．20％）、97．07％（RSD=0．48％）、98．39％（RSD=1．61％）。结论本法准确，重现性好，适用于银杏叶分散片中萜内酯的含量测定。Objective To establish a method for determination of terpene lactones in Ginkgo Leaves Dispersible Tablets. Methods The evaporation light scattering detector （ELSD） and ODS column （150 mm× 4.6 mm） were employed. The mobile phase consisted of propanol-tetrahydrofuran2water（1 ： 15 ： 84）. Results The logarithm value of sample size had a good linear relationship with the logarithm value of peak area when the sample size of bilobalide and ginkgolide A, B and C was 7.848-39. 240 μg, 3.8-19.0 μg, 7.08-35.40 μg, 3. 768- 18. 840 μg, respectively. The average recovery was 98.39% （RSD=0. 31%） ,98.41% （RSD=0. 20%）,97.07% （RSD=0. 48 %）, 98. 39 % （RSD= 1.61%）, respectively. Conclusion This method is accurate with good repeatability for the content determination of terpene lactones in Ginkgo Leaves Dispersible Tablets.

关 键 词：银杏叶 萜内酯 银杏内酯 白果内酯 含量测定 

分 类 号：R927.2[医药卫生—药学]