小鼠螺旋神经节神经元的分离和A型钾通道的电生理特性  

Dissociation of spiral ganglion neurons in postnatal mice and electrophysiological characteristics of its A-type potassium channels

作　　者：邓嘉虹[1] 蔡春春[1] 梁传余[2] 姜鹤群[1] 张林[1] 

机构地区：[1]云南省第一人民医院耳鼻喉科,昆明650032 [2]四川大学华西医院耳鼻喉科,成都610041

出　　处：《重庆医学》2010年第5期530-532,F0003,共4页Chongqing medicine

摘　　要：目的机械分离并酶解小鼠耳蜗螺旋神经节神经元(SGN)细胞,观察其A型钾通道的电生理特性。方法对生后1～6d的小鼠耳蜗螺旋神经节组织进行机械分离并酶解,对SGN进行免疫细胞化学染色鉴定,并利用全细胞膜片钳技术记录和分析A型钾通道电流。结果新生小鼠SGN在机械分离和酶解条件下,可以获得良好的细胞形态。SGN的A型钾通道在-60mV时激活,+40mV时失活,能被细胞外的4-氨基吡啶阻滞。结论成功建立了机械分离并酶解SGN细胞的方法,SGN的A型钾通道具有激活快、失活快的特点。Objective To mechanically dissociate and enzymolize spiral ganglion neurons （SGN） in postnatal mice and to observe the electrophysiological characteristics of those A-type potassium channels. Methods The mechanical dissociation and enzymolysis of spiral ganglion neurons were carried out with 1--6 d postnatal mice. Immunocytochemical stain identification was performed with fluorescent microscope. Record and analysis of A type potassium channels＇ currents were carried out with whole-cell patch clamp techniques. Results Spiral ganglion neurons of postnatal mice had good cell forms in the condition of mechanical dissociation and enzymolysis. The A-type potassium channels were activated in the membrane voltage of 60mv and inactivated in the membrane voltage of ＋40my, and were inhabited by extracellular 4-aminopyridine. Conclusion We successfully established the methods of mechanical dissociation and enzymolysis of SGN,the A-type potassium channels of SGN had the characties of quick activation and quick inactivation.

关 键 词：螺旋神经节神经元 免疫细胞化学 A型钾通道 

分 类 号：R764[医药卫生—耳鼻咽喉科]