加味补肝汤对糖尿病大鼠周围神经病变的保护作用及机制  

Protective action and mechanism of Jiaweibugan decoction on diabetic peripheral neuropathy rats

作　　者：陈泽奇[1] 李杰玉[1] 黄娟[1] 熊丽丽[1] 傅擎宇[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,长沙410008

出　　处：《山东医药》2010年第4期9-11,共3页Shandong Medical Journal

基　　金：湖南省卫生厅中医药科研经费资助项目(6202-38);湖南省重点学科建设经费资助项目[湘教通(2001)179号]

摘　　要：目的探讨加味补肝汤对实验性糖尿病大鼠周围神经病变的防治作用。方法以链脲佐菌素诱发糖尿病模型。分别于加味补肝汤治疗后4周、8周两个时间点处死大鼠,RT-PCR技术测定坐骨神经c-jun、蛋白激酶CβⅡ(PKC-βⅡ)mRNA的表达,免疫组织化学法测定脑源性神经生长因子(BDNF)蛋白的表达。结果加味补肝汤可降低c-jun、PKC-βⅡmRNA表达,上调BDNF蛋白表达(P<0.05)。结论加味补肝汤可减轻实验性糖尿病大鼠氧化应激反应,对糖尿病周围神经病变有较好的防治作用。Objective To explore the effect of Jiaweibugan decoction on peripheral neuropathy in experimental diabetic rats. Methods Forty diabetic rats were induced by 1% streptozotocin in which 20 rats were cured by Jiaweibugan decoction. Rats in each group were killed on 4th or 8th week after the beginning of treatment. The c-jun and PKC-β II mRNA of sciatic nerve were detected by using RT-PCR and the brain-derived neurotrophic factors （BDNF） protein of sciatic nerve was detected by method of immunohistochemistry assay. Results The expression of c-jun and PKC-β II in rats treated by Jiaweibugan decoction were lower than those in non-treated model rats （ P 〈 0.05 ）. The expression of BDNF protein in rats treated by Jiaweibugan decoction were higher than those in non-treated model rats （P 〈 0.05）. Conclusion Jiaweibugan decoction can reduce the oxidative stress reaction and has a preventive and therapeutic effect on peripheral neuropathy in experimental diabetic rats.

关 键 词：加味补肝汤 糖尿病神经病变 脑源性神经营养因子 C-JUN 蛋白激酶C 

分 类 号：R587.2[医药卫生—内分泌]