七带石斑鱼繁殖群体“突眼”症病原菌的分离与鉴定  

Isolation and identification of a pathogenic bacterium from Epinephelus septemfasciatus with exophthalmic disease

作　　者：陈超[1] 程波[2] 于宏[1] 王印庚[1] 杨志 史成银[1] 曲江波 赵丛明[4] 徐静荣 姜卓[5] 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛266071 [2]新疆农业大学动物科学学院,乌鲁木齐830052 [3]烟台技术开发区天源水产有限公司,265600 [4]天津立达海水资源开发有限公司,300381 [5]大连水产学院生命科学与技术学院,116023

出　　处：《渔业科学进展》2010年第1期25-33,共9页Progress in Fishery Sciences

基　　金：国家863计划项目(2006AA10A414-3);农业部948项目(2008-Z8)共同资助

摘　　要：2007-2008年间，对暴发“突眼”症的七带石斑鱼繁育群体，从细菌学、寄生虫学和病毒学等方面进行了全面的筛查和实验研究。从具有明显“突眼”症的七带石斑鱼病灶组织中分离出1株致病力极强的优势菌CB1008，经人工感染试验证实为此次七带石斑鱼“突眼”症的致病菌。该菌革兰氏染色呈阴性，电镜下观察菌体呈短杆状，极生单鞭毛。通过API-32E鉴定系统和菌体常规形态特征、培养特性和生理生化反应指标测定，以及16SrDNA测序分析等综合鉴定，菌株CB1008为弧菌属哈维氏弧菌Vibrioharveyi。药敏试验结果表明，该菌株对氯霉素、萘啶酸、四环素、氟哌酸和氟罗沙星等5种药物较为敏感。A pathogenic bacterial strain CB1008 was isolated from the eyes and internal or- gans of seven-band grouper Epinephelus septemfasciatus. It was proved to be the pathogen of the diseased seven-band grouper by artificial infection test. Morphological observations showed that it was a short-rod and Gram-negative bacterium with single polar flagella. According to API-32E system, through traditional physiological and biochemical experiments and 16S rDNA gene homology comparison of the bacterium, the results showed that the reisolated strain CB1008 was Vibrio harveyi. The test of sensitivity of strain CB1008 to 36 kinds of antibiotics revealed that the pathogen was sensitive to Chloramphenicol, Nalidixan, Tetracycline, Norflox- acin and Fleroxacin. V. harveyi is a kind of pathogenic bacterium commonly found in cultured marine {infish,but this is the first time that V. harveyi is reported as the pathogenic bacterium of seven-band grouper in China.

关 键 词：七带石斑鱼 “突眼”症 病原菌 哈维氏弧菌 

分 类 号：S941[农业科学—水产养殖]