波纹唇鱼染色体制备及核型的初步研究  

A preliminary study on chromosome preparation and karyotype of humphead wrasse Cheilinus undulatus

作　　者：周丽青[1] 杨爱国[1] 吴彪[1] 程鹏[1,2] 于宏[1] 王华 雷霁霖[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛266071 [2]大连水产学院,116023 [3]中国检验认证集团山东有限公司,青岛266071

出　　处：《渔业科学进展》2010年第1期54-58,共5页Progress in Fishery Sciences

基　　金：国家"863"计划项目(2006AA10A414)资助

摘　　要：出于对波纹唇鱼这一濒危物种资源调查和保护的目的,探讨了活体状况下波纹唇鱼染色体制备的方法,并对其染色体进行分析。剪取1龄波纹唇鱼的微量尾部鳍条及对应部位的愈合增生组织为材料,采用热滴片法制备染色体标本。核型分析结果表明,波纹唇鱼二倍体染色体数目2n=48,核型为2n=4M+10SM+32ST+2T,因所取鱼的条数有限,且均为雌性,所以尚不能确定是否有异型性染色体的存在。In order to protect the humphead wrasse Cheilinus undulatus （Ruppell, 1835）, an endangered species, the metaphase chromosome preparations of the live fish and its karyotype were studied in this paper. The metaphase chromosome was obtained from the fin and its heal- ing tissue of one-year old fish by hot air drying methods. The results showed that there were 48 chromosomes in diploid and their karyotype formulae was 2n=4M ＋10SM ＋ 32ST ＋2T. Heterotypic sex chromosome was not found since the number of the tested fish was limited and all the young fish were physiologically female.

关 键 词：波纹唇鱼 染色体 鳍条 愈合增生组织 核型 

分 类 号：Q959.403[生物学—动物学]