蝙蝠呼肠孤病毒感染BHK-21和Vero-E6细胞形态发生的比较观察  

Comparative studies of the morphogenesis of XJV in Vero-E6 and BHK-21 cells

作　　者：孟轲音[1] 杜林峰[1] 涂长春[1] 段铭[2] 邹啸环[1] 

机构地区：[1]军事医学科学院军事兽医研究所,吉林长春130062 [2]人兽共患病研究教育部重点实验室,吉林大学人兽共患病研究所,吉林长春130062

出　　处：《中国病原生物学杂志》2010年第2期84-85,110,F0003,共4页Journal of Pathogen Biology

摘　　要：目的通过对蝙蝠呼肠孤病毒在BHK-21和Vero-E6细胞形态发生的比较研究,筛选出该病毒的最佳宿主细胞。方法将感染呼肠孤病毒XJV株的BHK-21和Vero-E6细胞用戊二醛、锇酸固定,制成超薄切片,用透射电镜观察。同时测定病毒滴度。结果XJV在2种细胞中的适应程度不同,XJV更适于在BHK-21细胞中增殖。XJV在2种细胞中的形态发生不同,在BHK-21细胞中形成的包涵体呈典型的晶格状排列,在Vero-E6细胞中形成的包涵体呈杂乱的球状。XJV用BHK-21和Vero-E6细胞育传5代,病毒滴度分别为105.5TCID50/ml和104.5TCID50/ml。结论BHK-21细胞是蝙蝠呼肠孤病毒XJV株的最佳宿主细胞。Objective The morphogenesis of an XJV strain in BHK 21and Vero-E6 cells was comparatively studied to select the best host cells. Methods BHK-21 and Vero-E6 cells were infected with the XJV strain and treated according to methods for preparation and observation of specimens using transmission electron microscopy. Results The adaptation of the XJV strain differed in two kinds of cells, and BHK-21 cells were more suitable for XJV strain proliferation. The morphogenesis of the XJV strain also differed in two kinds of cells. In the BHK-21 cells, the XJV inclusions were ar ranged in a crystal lattice, but forms in the Vero-E6 cells were disordered spheres. The viral titer in the BHK-21 cells was higher than in the Vero-E6 cells. Conclusion BHK 21 cells are the best host cells for XJV.

关 键 词：呼肠孤病毒 BHK-21细胞 Vero—E6细胞 形态发生 透射电子显微镜 

分 类 号：S718.85[农业科学—林学]