日本血吸虫生殖相关蛋白SjWD40的虫体免疫定位  

Immunolocalization of the proliferation-related protein WD40 of Schistosoma japonicum

作　　者：刘玲[1] 陈守义[2] 黄灿[1] 王乐旬[1] 徐劲[1] 

机构地区：[1]中山大学中山医学院寄生虫学教研室,广东广州510080 [2]广州市疾病预防控制中心,广东广州510080

出　　处：《中国病原生物学杂志》2010年第2期118-119,133,F0002,共4页Journal of Pathogen Biology

摘　　要：目的研究抗重组日本血吸虫生殖相关蛋白WD40(SjWD40)多克隆抗体对天然SjWD40的结合活性,观察SjWD40在虫体内的定位。方法从阳性钉螺体内逸出尾蚴,感染家兔,于感染后42 d剖杀收集成虫和虫卵制备石蜡切片,利用抗重组蛋白SjWD40多克隆抗体,通过间接免疫荧光方法探讨SjWD40在虫体内的分布。结果SjWD40广泛分布于雌虫的卵黄腺、雄虫的睾丸,虫卵的毛蚴体壁,尾蚴的头腺、钻腺、腺管等部位。结论免疫荧光染色法确定SjWD40蛋白主要分布于血吸虫成虫的生殖腺、毛蚴体壁及尾蚴腺体等组织。Objective To study the localization of the proliferation-related protein WD40 of Schistosozna japonicuzn （SjWD40） in the parasite. Methods Cercariae were collected from infected Oncomelania snails. Adult worms and eggs of S. japonicuzn were obtained from infected rabbits 42 days post-infection. The whole cercaria and paraffin sections of adult worm and eggs of S. japonicum were studied by immunohistochemical staining with anti-rSjWD40 serum as the first antibody. Results SjWD40 was expressed in the vitelline glands and testes of adult female and male worms, in the integument of miracidia in eggs, and in the cephalic glands and penetration glands and ducts of cercariae of S. japonicum under natural conditions. Conclusion The wide distribution and extensive sites of SjWD40 in the parasite were clearly demonstrated, helping to provide significant clues for further studies of the biological action and use of WD40 protein.

关 键 词：血吸虫 日本 SjWD40 多克隆抗体 间接免疫荧光试验 定位 

分 类 号：R383.24[医药卫生—医学寄生虫学]