川金丝猴mtDNA D-loop序列遗传多态性分析  

Analysis on Mitochondrial DNA D-loop Sequences Genetic Polymorphism of Rhinopithecus roxellana

作　　者：何丽[1] 张于光[2] 李迪强[2] 李大全[1] 

机构地区：[1]新疆石河子大学动物科技学院,石河子832000 [2]中国林业科学研究院森林生态环境与保护研究所国家林业局森林生态环境重点实验室,北京100091

出　　处：《动物学杂志》2010年第1期70-76,共7页Chinese Journal of Zoology

基　　金：国家"十一五"科技支撑计划项目(No.2006BAD03A1202;2007BAC03A08-5);国家科技基础条件平台建设项目(No.2005DKA21404)

摘　　要：采用非损伤性DNA分析技术,分析了甘肃白水江保护区、陕西长青保护区、湖北神农架保护区3个川金丝猴(Rhinopithecus roxellana)种群中的20份粪便样品和2份肌肉样品,成功扩增了mtDNA D-loop区部分片段。经过GenBank数据库的BLAST比对,确定了22份样品均来自川金丝猴,经过Clustal W和DNASP软件分析,在22份川金丝猴mtDNA D-loop区393bp中,共检测出54个多态性位点,分为17个单倍型,单倍型多态性(h)为0.965,核苷酸多态性(π)为3.10%。3个种群之间的遗传距离为0.003～0.098,核苷酸差异为0.08%～2.80%,表明所得到的川金丝猴样品中存在着较丰富的遗传多态性,种群间存在一定的遗传差异。By using noninvasive DNA technology,20 fecal samples and 2 muscle samples of Sichuan Snub-nosed Monkeys（Rhinopithecus roxellana）from Baishuijiang Nature Reserve of Gansu province （BSJ）, Changqing Nature Reserve of Shaanxi Province （SH） and Shennongjia Nature Reserve of Hubei Province （SNJ） were analyzed and the mitochondrial DNA D-loop fragments were successfully amplified. Sequences were aligned with the GenBank by BLAST software and 22 samples were identified as R. roxellana. According to the analysis by Clustal W and DNASP software, 17 haplotypes and 54 diversity sites were found in 22 Sichuan snub-nosed monkey samples with 393 bp length of mtDNA D-loop region. The haplotype diversity was 0. 965 and nucleotide diversity was 3. 10%. The genetic distance and nucleotide divergence of R. roxellana among BSJ, SH, and SNJ were 0. 003 - 0. 098 and 0.08% - 2. 80% , respectively. Therefore, the results show a high variation in the mitochondrial DNA D-loop region and genetic distance of R. roxellana from the three sampling sites.

关 键 词：川金丝猴 线粒体D-LOOP区 单倍型 遗传多样性 

分 类 号：Q953[生物学—动物学]